YAO is a nucleolar WD40-repeat protein critical for embryogenesis and gametogenesis in Arabidopsis.
YAO is a nucleolar WD40-repeat protein critical for embryogenesis and gametogenesis in Arabidopsis.
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YAO 是一种核仁 WD40 重复蛋白，对拟南芥胚胎发生和配子发生至关重要
DOI：
10.1186/1471-2229-10-169
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发表时间：
2010-08-11
影响因子：
5.3
通讯作者：
Yang WC
中科院分区：
文献类型：
--
作者：
Li HJ;Liu NY;Shi DQ;Liu J;Yang WC
在开花植物中，配子发生产生多细胞的雄配子体和雌配子体。在模式系统拟南芥中，雄配子体或花粉粒含有两个精细胞和一个营养细胞。雌配子体或胚囊含有7个细胞，即1个卵、2个助细胞、1个中央细胞和3个反足细胞。中央细胞和卵的双受精分别产生三倍体胚乳和二倍体合子，后者进一步发育成胚。早期胚胎模式的遗传控制，特别是第一次合子分裂的启动和细胞板的定位，在很大程度上尚不清楚。结果在这里，我们报告了一种突变yaozhe（yao）的特征，该突变导致受精卵停滞和受精卵细胞板的错位，导致早期胚胎死亡。此外，配子体发育部分受损。一小部分突变胚囊停滞在四核期，核定位异常。YAO基因编码一个含有7个WD重复序列的核仁蛋白。YAO在人和酵母中的同源物是U3 snoRNP复合物的组成部分，在18SrRNA加工中起作用。YAO在细胞分裂活跃的组织中广泛表达，包括胚囊、花粉、胚胎、结论表型分析表明，YAO是第一次合子分裂面正确定位所必需的，在胚乳和根尖中起着关键作用。拟南芥配子发生由于YAO是一种核仁蛋白，其在酵母和人类中的对应物是U3 snoRNP复合物的组分，因此我们推测YAO也很可能参与植物中的rRNA加工。
BackgroundIn flowering plants, gametogenesis generates multicellular male and female gametophytes. In the model system Arabidopsis, the male gametophyte or pollen grain contains two sperm cells and a vegetative cell. The female gametophyte or embryo sac contains seven cells, namely one egg, two synergids, one central cell and three antipodal cells. Double fertilization of the central cell and egg produces respectively a triploid endosperm and a diploid zygote that develops further into an embryo. The genetic control of the early embryo patterning, especially the initiation of the first zygotic division and the positioning of the cell plate, is largely unknown.ResultsHere we report the characterization of a mutation,yaozhe (yao), that causes zygote arrest and misplacement of cell plate of the zygote, leading to early embryo lethality. In addition, gametophyte development is partially impaired. A small portion of the mutant embryo sacs are arrested at four-nucleate stage with aberrant nuclear positioning. Furthermore, the competence of male gametophytes is also compromised.YAOencodes a nucleolar protein with seven WD-repeats. Its homologues in human and yeast have been shown to be components of the U3 snoRNP complex and function in 18S rRNA processing.YAOis expressed ubiquitously, with high level of expression in tissues under active cell divisions, including embryo sacs, pollen, embryos, endosperms and root tips.ConclusionsPhenotypic analysis indicated thatYAOis required for the correct positioning of the first zygotic division plane and plays a critical role in gametogenesis in Arabidopsis. Since YAO is a nucleolar protein and its counterparts in yeast and human are components of the U3 snoRNP complex, we therefore postulate that YAO is most likely involved in rRNA processing in plants as well.
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影响因子：
7
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