Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages.
Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages.
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DOI：
10.3389/fmicb.2021.770499
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发表时间：
2021
影响因子：
5.2
通讯作者：
Manning SD
中科院分区：
文献类型：
--
作者：
McCutcheon CR;Pell ME;Gaddy JA;Aronoff DM;Petroff MG;Manning SD
虽然新生儿和胎儿病原体B组链球菌(Gbs)无症状地定植在30%的∼孕妇的阴道内，但她们的后代中只有一小部分发展为侵袭性疾病。我们和其他人推测，这些二态临床表型是由菌株变异性驱动的；然而，促进这些不同临床表型的细菌因素仍不清楚。先前的研究表明，GBS产生膜泡(MVS)，其中含有能够诱导不良妊娠结局的活性毒力因子。由于毒株变异与囊泡成分或产量之间的关系尚不清楚，我们试图对代表三个系统发育不同谱系的不同临床GBS毒株产生的MV进行量化，并检测其蛋白质组成。我们发现不同菌株的MV产量不同，某些菌株的产量几乎是其他菌株的两倍。蛋白质组的系统聚类和主成分分析表明，MV的组成与谱系有关，但与临床表型无关。参与毒力或免疫调节的多种蛋白质，包括透明质酸酶、C5a多肽酶和唾液酸酶，在MVS中差异丰富，这是这种差异的部分原因。综上所述，这些数据表明，GBS MVS的产生和组成以一种依赖于菌株的方式变化，表明MVS具有与毒力相关的谱系特异性功能。这些差异可能有助于在感染代表不同血统的GBS菌株的个体中观察到临床表型的差异。
Although the neonatal and fetal pathogen Group B Streptococcus (GBS) asymptomatically colonizes the vaginal tract of ∼30% of pregnant women, only a fraction of their offspring develops invasive disease. We and others have postulated that these dimorphic clinical phenotypes are driven by strain variability; however, the bacterial factors that promote these divergent clinical phenotypes remain unclear. It was previously shown that GBS produces membrane vesicles (MVs) that contain active virulence factors capable of inducing adverse pregnancy outcomes. Because the relationship between strain variation and vesicle composition or production is unknown, we sought to quantify MV production and examine the protein composition, using label-free proteomics on MVs produced by diverse clinical GBS strains representing three phylogenetically distinct lineages. We found that MV production varied across strains, with certain strains displaying nearly twofold increases in production relative to others. Hierarchical clustering and principal component analysis of the proteomes revealed that MV composition is lineage-dependent but independent of clinical phenotype. Multiple proteins that contribute to virulence or immunomodulation, including hyaluronidase, C5a peptidase, and sialidases, were differentially abundant in MVs, and were partially responsible for this divergence. Together, these data indicate that production and composition of GBS MVs vary in a strain-dependent manner, suggesting that MVs have lineage-specific functions relating to virulence. Such differences may contribute to variation in clinical phenotypes observed among individuals infected with GBS strains representing distinct lineages.
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DOI：
10.1038/nrmicro3480
发表时间：
2015-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Brown L;Wolf JM;Prados-Rosales R;Casadevall A
通讯作者：
Casadevall A
DOI：
10.1007/978-1-62703-245-2_16
发表时间：
2013
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Chutkan, Halima;Macdonald, Ian;Manning, Andrew;Kuehn, Meta J
通讯作者：
Kuehn, Meta J
影响因子：
3.1
作者：
Korir, Michelle L.;Knupp, David;Manning, Shannon D.
通讯作者：
Manning, Shannon D.
影响因子：
6.4
作者：
Armistead, Blair;Quach, Phoenicia;Rajagopal, Lakshmi
通讯作者：
Rajagopal, Lakshmi
影响因子：
11.8
作者：
Manning, Shannon D.;Lewis, Maggi A.;Davies, H. Dele
通讯作者：
Davies, H. Dele