Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma.
Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma.
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DOI：
10.1155/2016/9343460
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发表时间：
2016
影响因子：
4.6
通讯作者：
Murata M
中科院分区：
文献类型：
--
作者：
Wang S;Ma N;Zhao W;Midorikawa K;Kawanishi S;Hiraku Y;Oikawa S;Zhang Z;Huang G;Murata M
硝化性和氧化性DNA损伤在炎症相关癌症的发生中起着重要作用。为探讨干细胞在EB病毒感染相关性鼻咽癌中的作用，我们采用双重免疫荧光染色方法检测了鼻咽癌组织和鼻咽癌细胞系中多种肿瘤干/祖细胞标志物(CD44v6、CD24和ALDH1A1)的表达。我们还测量了8-硝基鸟嘌呤的形成作为炎症相关DNA损伤的指标。鼻咽癌组织间质中的癌细胞和炎性细胞的8-硝基鸟嘌呤染色强度明显高于慢性鼻咽炎组织。CD44v6和ALDH1A1在原发鼻咽癌组织中的表达水平显著高于慢性鼻咽炎组织。同样，CD44v6和ALDH1A1在鼻咽癌细胞系中的表达比在永生化的鼻咽上皮细胞系中更强。CD24染色在鼻咽癌和慢性鼻咽炎组织中的表达差异无统计学意义。在鼻咽癌组织中CD44v6和ALDH1A1阳性干细胞中均检测到8-硝基鸟嘌呤。综上所述，CD44v6和ALDH1A1是鼻咽癌的候选干细胞标记物，炎症导致DNA损伤的增加可能导致干细胞突变，从而导致鼻咽癌的发生。
Nitrative and oxidative DNA damage plays an important role in inflammation-related carcinogenesis. To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. We also measured 8-nitroguanine formation as an indicator of inflammation-related DNA lesions. The staining intensity of 8-nitroguanine was significantly higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. Expression levels of CD44v6 and ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues. Similarly, more intense staining of CD44v6 and ALDH1A1 was detected in an NPC cell line than in an immortalized nasopharyngeal epithelial cell line. In the case of CD24 staining, there was no significant difference between NPC and chronic nasopharyngitis tissues. 8-Nitroguanine was detected in both CD44v6- and ALDH1A1-positive stem cells in NPC tissues. In conclusion, CD44v6 and ALDH1A1 are candidate stem cell markers for NPC, and the increased formation of DNA lesions by inflammation may result in the mutation of stem cells, leading to tumor development in NPC.
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影响因子：
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