Effects of phonation time and magnitude dose on vocal fold epithelial genes, barrier integrity, and function.
Effects of phonation time and magnitude dose on vocal fold epithelial genes, barrier integrity, and function.
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DOI：
10.1002/lary.24827
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发表时间：
2014-12
期刊：
影响因子：
2.6
通讯作者：
Rousseau, Bernard
中科院分区：
文献类型：
--
作者：
Kojima, Tsuyoshi;Valenzuela, Carla V.;Novaleski, Carolyn K.;Van Deusen, Mark;Mitchell, Joshua R.;Garrett, C. Gaelyn;Sivasankar, M. Preeti;Rousseau, Bernard
研究振动暴露时间和强度的增加对声带连接蛋白转录、结构改变和功能组织结果的影响。动物实验。对100只新西兰白色种兔进行了研究。测量因变量响应于振动暴露的增加的时间剂量（30、60或120分钟）和幅度剂量（控制、模态强度和升高的强度）。检测闭合蛋白、闭合小带1（ZO-1）、E-钙粘蛋白、β-连环蛋白、白细胞介素1β（IL-1β）、环氧合酶2（考克斯-2）、转化生长因子β 1（TGFβ1）和纤维连接蛋白的mRNA表达。使用透射电子显微镜（TEM）评估组织结构变化。跨上皮电阻用于测量功能组织结果。Occludin基因表达下调声带暴露于120分钟的时间剂量的提高强度发声，相对于控制，模态强度发声。ZO-1基因表达上调后120分钟的时间剂量的模态强度发声，与对照组相比，和下调后120分钟的时间剂量的提高强度发声，与模态强度发声。与控制和模态强度发声相比，120分钟时间剂量的提高强度发声后，E-cadherin基因表达下调。透射电镜显示，随着振动暴露时间和强度的增加，分层鳞状上皮细胞广泛脱落。与所有其他组相比，在暴露于提高强度发声的组织中进行了较低的跨上皮电阻测量的一般观察。这项研究提供了证据声带组织的反应，以不同的时间和幅度剂量的振动暴露。N/A
To investigate the effects of increasing time and magnitude doses of vibration exposure on transcription of the vocal fold's junctional proteins, structural alterations, and functional tissue outcomes. Animal study. 100 New Zealand White breeder rabbits were studied. Dependent variables were measured in response to increasing time doses (30, 60, or 120 minutes) and magnitude doses (control, modal intensity, and raised intensity) of vibration exposure. Messenger RNA expression of occludin, zonula occluden-1 (ZO-1), E-cadherin, β-catenin, interleukin 1β (IL-1β), cyclooxygenase-2 (COX-2), transforming growth factor β-1 (TGFβ1), and fibronectin were measured. Tissue structural alterations were assessed using transmission electron microscopy (TEM). Transepithelial resistance was used to measure functional tissue outcomes. Occludin gene expression was downregulated in vocal folds exposed to 120 minute time doses of raised intensity phonation, relative to control, and modal intensity phonation. ZO-1 gene expression was upregulated following a 120 minute time dose of modal intensity phonation, compared to control, and downregulated after a 120 minute time dose of raised intensity phonation, compared to modal intensity phonation. E-cadherin gene expression was downregulated after a120 minute time dose of raised intensity phonation, compared to control and modal intensity phonation. TEM revealed extensive desquamation of the stratified squamous epithelial cells with increasing time and magnitude doses of vibration exposure. A general observation of lower transepithelial resistance measures was made in tissues exposed to raised intensity phonation, compared to all other groups. This study provides evidence of vocal fold tissue responses to varying time and magnitude doses of vibration exposure. N/A
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DOI：
10.1177/0194599811428273
发表时间：
2012-02
期刊：
Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery
影响因子：
--
作者：
Erickson-Levendoski E;Sivasankar MP
通讯作者：
Sivasankar MP
影响因子：
1.4
作者：
Ge, Ping Jiang;French, Lesley C.;Rousseau, Bernard
通讯作者：
Rousseau, Bernard
影响因子：
4.3
作者：
Chang Z;Kishimoto Y;Hasan A;Welham NV
通讯作者：
Welham NV
影响因子：
3.4
作者：
Kimura, Kazuhiro;Teranishi, Shinichiro;Nishida, Teruo
通讯作者：
Nishida, Teruo
影响因子：
3.3
作者：
Katsuno, Tatsuya;Umeda, Kazuaki;Tsukita, Sachiko
通讯作者：
Tsukita, Sachiko