Tracking intracellular uptake and localisation of alkyne tagged fatty acids using Raman spectroscopy.
Tracking intracellular uptake and localisation of alkyne tagged fatty acids using Raman spectroscopy.
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DOI：
10.1016/j.saa.2018.01.064
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发表时间：
2018-05-15
期刊：
影响因子：
--
通讯作者：
Graham D
中科院分区：
文献类型：
--
作者：
Jamieson LE;Greaves J;McLellan JA;Munro KR;Tomkinson NCO;Chamberlain LH;Faulds K;Graham D
脂质的细胞内摄取、分布和代谢是健康细胞中受到严格调控的特征。为了更好地了解这些特征在疾病期间如何被破坏，非常需要一种能够以微创方式以高水平的物种特异性理解这些特征的分析技术。在这项研究中，三种不同的炔标记的脂肪酸在单细胞中的摄取和分布进行了监测和比较，突出了拉曼光谱结合炔标记的能力，更好地了解的精细细节方面的摄取，分布和代谢的非常化学特定的脂质种类。这表明直接使用带有炔标记的脂质的拉曼光谱进行细胞研究的前景，而不是随后将荧光团点击到炔上进行荧光成像。通过拉曼光谱法检测三种不同的炔标记的脂肪酸。
Intracellular uptake, distribution and metabolism of lipids are tightly regulated characteristics in healthy cells. An analytical technique capable of understanding these characteristics with a high level of species specificity in a minimally invasive manner is highly desirable in order to understand better how these become disrupted during disease. In this study, the uptake and distribution of three different alkyne tagged fatty acids in single cells were monitored and compared, highlighting the ability of Raman spectroscopy combined with alkyne tags for better understanding of the fine details with regard to uptake, distribution and metabolism of very chemically specific lipid species. This indicates the promise of using Raman spectroscopy directly with alkyne tagged lipids for cellular studies as opposed to subsequently clicking of a fluorophore onto the alkyne for fluorescence imaging. Three different alkyne tagged fatty acids detected by Raman spectroscopy Relative intracellular uptake of each determined Intracellular localisation with subcellular resolution determined
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