Neurotransmitters and neuropeptides in gonadal steroid receptor-expressing cells in medial preoptic area subregions of the male mouse.
Neurotransmitters and neuropeptides in gonadal steroid receptor-expressing cells in medial preoptic area subregions of the male mouse.
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男性小鼠内侧前区域的性腺类固醇受体表达细胞中的神经递质和神经肽。
DOI：
10.1038/s41598-017-10213-4
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发表时间：
2017-08-29
影响因子：
4.6
通讯作者：
Funato H
中科院分区：
文献类型：
--
作者：
Tsuneoka Y;Yoshida S;Takase K;Oda S;Kuroda M;Funato H
睾酮通过在脑中表达的雄激素受体（AR）和雌激素受体（ER）α参与男性性行为、父母行为和攻击行为。尽管一些研究已经证明内侧视前区（MPOA）中的ER α和AR是雄性小鼠表现性行为和攻击行为所必需的，但小鼠MPOA中ER α和AR表达细胞的分子特征在很大程度上是未知的。在这里，我们进行了原位杂交的神经递质和神经肽，结合免疫组化ER α和AR的定量和特征的雄性小鼠的MPOA亚区的性腺类固醇受体表达细胞。强啡肽原、前脑啡肽原（Penk）、可卡因和苯丙胺相关转录物、神经降压素、甘丙肽、速激肽（Tac）1、Tac 2和促甲状腺激素释放激素（Trh）在MPOA亚区具有不同的表达模式。在整个MPOA中，Gad67表达细胞是ER α和AR表达细胞中最主要的神经元亚型。ER α和AR免疫反应的百分比根据神经元亚型而变化。在MPOA中，相当比例的神经降压素、甘丙肽、Tac2和Penk表达细胞为ER α和AR阳性，而绝大多数Trh表达细胞为阴性。这些结果表明，睾酮发挥不同的影响取决于神经元亚型和MPOA亚区。
Testosterone is involved in male sexual, parental and aggressive behaviors through the androgen receptor (AR) and estrogen receptor (ER) α expressed in the brain. Although several studies have demonstrated that ERα and AR in the medial preoptic area (MPOA) are required for exhibiting sexual and aggressive behaviors of male mice, the molecular characteristics of ERα- and AR-expressing cells in the mouse MPOA are largely unknown. Here, we performed in situ hybridization for neurotransmitters and neuropeptides, combined with immunohistochemistry for ERα and AR to quantitate and characterize gonadal steroid receptor-expressing cells in the MPOA subregions of male mice. Prodynorphin, preproenkephalin (Penk), cocaine- and amphetamine-related transcript, neurotensin, galanin, tachykinin (Tac)1, Tac2 and thyrotropin releasing hormone (Trh) have distinct expression patterns in the MPOA subregions. Gad67-expressing cells were the most dominant neuronal subtype among the ERα- and AR-expressing cells throughout the MPOA. The percentage of ERα- and AR-immunoreactivities varied depending on the neuronal subtype. A substantial proportion of the neurotensin-, galanin-, Tac2- and Penk-expressing cells in the MPOA were positive for ERα and AR, whereas the vast majority of the Trh-expressing cells were negative. These results suggest that testosterone exerts differential effects depending on both the neuronal subtypes and MPOA subregions.
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影响因子：
2.5
作者：
Creutz, LM;Kritzer, MF
通讯作者：
Kritzer, MF
影响因子：
4.7
作者：
Dobolyi, Arpad
通讯作者：
Dobolyi, Arpad
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29
作者：
Li, Zhiying;Kelly, Leah;Friedman, Jeffrey Michael
通讯作者：
Friedman, Jeffrey Michael
影响因子：
4.6
作者：
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通讯作者：
Braun, RE
影响因子：
4.8
作者：
Howard, G;Peng, LH;Hyde, JF
通讯作者：
Hyde, JF