Zymoseptoria tritici white-collar complex integrates light, temperature and plant cues to initiate dimorphism and pathogenesis.
Zymoseptoria tritici white-collar complex integrates light, temperature and plant cues to initiate dimorphism and pathogenesis.
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DOI：
10.1038/s41467-022-33183-2
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发表时间：
2022-09-26
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
由孢子向菌丝的转化是植物病原真菌发酵壳孢菌入侵寄主的关键。这种二态转换可以通过体外高温（约27 °C）启动;然而，这种条件可能会诱导细胞热应激，质疑其与田间感染的相关性。在这里，我们研究了温度和其他因素对双态开关的调节。来自小麦种植区的气候数据表明，病原体在夏季偶尔会经历高温，如27 °C。然而，在四个野生型菌株中使用荧光双态转换报告基因（FDR 1），我们表明双态转换已经在15-18 °C开始，并且通过小麦叶表面化合物增强。转录组学揭示了1261个基因在所有菌株的菌丝中上调或下调。这些泛株核心二型性基因（PCDG）编码已知的效应子、二型性和转录因子以及光响应蛋白（天鹅绒因子、视蛋白、推定的蓝光受体）。基于FDR 1的遗传筛选揭示了白领复合体（WCC）在二态性和毒力中的关键作用，其通过控制PCDG表达来介导。因此，WCC将光与生物和非生物线索整合以协调Z。感染性三联症从孢子到菌丝的转变是植物病原真菌Zymoseptoria triangulae入侵寄主的关键。在这里，作者表明，孢子到菌丝的转变是由小麦叶表面化合物增强，并由白领复合物调节，它将光与生物和非生物的线索，让主机入侵通过开放的气孔。
Transitioning from spores to hyphae is pivotal to host invasion by the plant pathogenic fungus Zymoseptoria tritici. This dimorphic switch can be initiated by high temperature in vitro (~27 °C); however, such a condition may induce cellular heat stress, questioning its relevance to field infections. Here, we study the regulation of the dimorphic switch by temperature and other factors. Climate data from wheat-growing areas indicate that the pathogen sporadically experiences high temperatures such as 27 °C during summer months. However, using a fluorescent dimorphic switch reporter (FDR1) in four wild-type strains, we show that dimorphic switching already initiates at 15–18 °C, and is enhanced by wheat leaf surface compounds. Transcriptomics reveals 1261 genes that are up- or down-regulated in hyphae of all strains. These pan-strain core dimorphism genes (PCDGs) encode known effectors, dimorphism and transcription factors, and light-responsive proteins (velvet factors, opsins, putative blue light receptors). An FDR1-based genetic screen reveals a crucial role for the white-collar complex (WCC) in dimorphism and virulence, mediated by control of PCDG expression. Thus, WCC integrates light with biotic and abiotic cues to orchestrate Z. tritici infection. Transitioning from spores to hyphae is crucial for host invasion by the plant pathogenic fungus Zymoseptoria tritici. Here, the authors show that the spore-to-hypha transition is enhanced by wheat leaf surface compounds and is regulated by the white-collar complex, which integrates light with biotic and abiotic cues to allow host invasion through open stomata.
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影响因子：
4.4
作者：
Choi, Yoon-E;Goodwin, Stephen B.
通讯作者：
Goodwin, Stephen B.
影响因子：
3
作者：
Ben M'Barek, Sarrah;Cordewener, Jan H. G.;Kema, Gerrit H. J.
通讯作者：
Kema, Gerrit H. J.
DOI：
10.1534/g3.115.017731
发表时间：
2015-04-27
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Grandaubert J;Bhattacharyya A;Stukenbrock EH
通讯作者：
Stukenbrock EH
DOI：
10.1016/j.bbamcr.2014.10.005
发表时间：
2015-01-01
影响因子：
5.1
作者：
Camoes, Fatima;Islinger, Markus;Schrader, Michael
通讯作者：
Schrader, Michael
影响因子：
4.4
作者：
Albrecht D;Guthke R;Brakhage AA;Kniemeyer O
通讯作者：
Kniemeyer O