Integrative analysis of the heat shock response in Aspergillus fumigatus.
Integrative analysis of the heat shock response in Aspergillus fumigatus.
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DOI：
10.1186/1471-2164-11-32
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发表时间：
2010-01-15
期刊：
影响因子：
4.4
通讯作者：
Kniemeyer O
中科院分区：
文献类型：
--
作者：
Albrecht D;Guthke R;Brakhage AA;Kniemeyer O
烟曲霉是一种耐热的人类致病性霉菌，是免疫功能低下患者侵袭性曲霉病（IA）的最常见原因。它的优势是基于几个因素，其中大多数仍然是未知的。对A.烟曲霉是已被指定为致病性的性状之一。它允许真菌在高达或高于发热人类宿主的温度下生长。为了阐明其耐热机理，我们分析了热胁迫下A.通过二维荧光差异凝胶电泳（DIGE）分析烟曲霉蛋白质组在从30°C到48°C的温度变化期间的变化。为了改善2D凝胶图像分析结果，通过缺失值填补和归一化优化蛋白质斑点定量。差异调节的蛋白质与先前发表的A.烟熏。这项研究是由生物信息学分析的转录因子结合位点（TFBS）在启动子区的基因，其相应的蛋白质差异调节热休克。通过质谱（MS）鉴定了91个差异调节的蛋白质点，代表64种不同的蛋白质。它们表现出连续的上升、下降或振荡调节。许多已鉴定的蛋白质参与蛋白质折叠（分子伴侣）、氧化应激反应、信号转导、转录、翻译、碳水化合物和氮代谢。转录水平的改变和相应的蛋白质之间的相关性检测到的差异调节蛋白质的一半。有趣的是，一些以前未描述的热休克调节因子Hsf 1的推定目标被确定。这为Hsf 1依赖性调节甘露醇的合成、翻译、细胞骨架动力学和细胞分裂提供了证据。烟熏。此外，启动子的计算分析揭示了一些热休克诱导基因上游的AP-2 α样转录因子的假定结合位点。到目前为止，这种因子只在脊椎动物中发现。我们新建立的DIGE数据分析工作流程提高了数据质量，并广泛适用于其他DIGE数据集。我们的研究结果表明，热休克反应在A。烟曲霉菌不同于已经充分研究的酵母菌和其它丝状真菌。
Aspergillus fumigatus is a thermotolerant human-pathogenic mold and the most common cause of invasive aspergillosis (IA) in immunocompromised patients. Its predominance is based on several factors most of which are still unknown. The thermotolerance of A. fumigatus is one of the traits which have been assigned to pathogenicity. It allows the fungus to grow at temperatures up to and above that of a fevered human host. To elucidate the mechanisms of heat resistance, we analyzed the change of the A. fumigatus proteome during a temperature shift from 30°C to 48°C by 2D-fluorescence difference gel electrophoresis (DIGE). To improve 2D gel image analysis results, protein spot quantitation was optimized by missing value imputation and normalization. Differentially regulated proteins were compared to previously published transcriptome data of A. fumigatus. The study was augmented by bioinformatical analysis of transcription factor binding sites (TFBSs) in the promoter region of genes whose corresponding proteins were differentially regulated upon heat shock. 91 differentially regulated protein spots, representing 64 different proteins, were identified by mass spectrometry (MS). They showed a continuous up-, down- or an oscillating regulation. Many of the identified proteins were involved in protein folding (chaperones), oxidative stress response, signal transduction, transcription, translation, carbohydrate and nitrogen metabolism. A correlation between alteration of transcript levels and corresponding proteins was detected for half of the differentially regulated proteins. Interestingly, some previously undescribed putative targets for the heat shock regulator Hsf1 were identified. This provides evidence for Hsf1-dependent regulation of mannitol biosynthesis, translation, cytoskeletal dynamics and cell division in A. fumigatus. Furthermore, computational analysis of promoters revealed putative binding sites for an AP-2alpha-like transcription factor upstream of some heat shock induced genes. Until now, this factor has only been found in vertebrates. Our newly established DIGE data analysis workflow yields improved data quality and is widely applicable for other DIGE datasets. Our findings suggest that the heat shock response in A. fumigatus differs from already well-studied yeasts and other filamentous fungi.
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影响因子：
3
作者：
Chang, YC;Tsai, HF;Kwon-Chung, KJ
通讯作者：
Kwon-Chung, KJ
影响因子：
3.6
作者：
Boy-Marcotte, E;Lagniel, G;Labarre, J
通讯作者：
Labarre, J
影响因子：
3.3
作者：
Chen, DR;Toone, WM;Bähler, J
通讯作者：
Bähler, J
DOI：
10.1017/s0016672307008476
发表时间：
2006-12-01
期刊：
GENETICAL RESEARCH
影响因子：
--
作者：
Altman, Naomi S.;Hua, Jun
通讯作者：
Hua, Jun
影响因子：
5.8
作者：
Fodor, IK;Nelson, DO;McCutchen-Maloney, SL
通讯作者：
McCutchen-Maloney, SL