Alcelaphine Herpesvirus-1 (Malignant Catarrhal Fever Virus) in Wildebeest Placenta: Genetic Variation of ORF50 and A9.5 Alleles.
Alcelaphine Herpesvirus-1 (Malignant Catarrhal Fever Virus) in Wildebeest Placenta: Genetic Variation of ORF50 and A9.5 Alleles.
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叶贝斯特胎盘中的Alcelaphine疱疹病毒-1（恶性CATARRHAL热病毒）：ORF50和A9.5等位基因的遗传变异。
DOI：
10.1371/journal.pone.0124121
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Russell GC
中科院分区：
文献类型：
--
作者：
Lankester F;Lugelo A;Mnyambwa N;Ndabigaye A;Keyyu J;Kazwala R;Grant DM;Relf V;Haig DM;Cleaveland S;Russell GC
在牛羚胎盘组织中首次检出牛恶性卡他热病原体AlHV-1。虽然病毒载量很低，但在94个测试样本中超过50%的病毒DNA的发现支持了胎盘组织可能在疾病传播中发挥作用的可能性，以及角马小牛在子宫内感染的可能性。对两个病毒基因座进行测序，以检测从角马和牛中获得的病毒样本之间的差异：编码裂解循环反激活蛋白的ORF50基因和编码一种新型多态病毒糖蛋白的A9.5基因。ORF50保守性较好，新发现的6个等位基因仅在一个或两个碱基位置上存在差异。相比之下，虽然只发现了3个新的A9.5等位基因，但这些等位基因在核苷酸水平上的差异高达13%，在氨基酸水平上的差异高达20%。本研究中确定的额外A9.5序列的结构同源性搜索为最近的分析增加了力量，确定了四螺旋束细胞因子白介素-4 （IL4）是主要的同源物。从坦桑尼亚牛和角马获得的大多数MCF病毒样本编码的A9.5多肽与实验室维持的AlHV-1 C500株中先前表征的A9.5等位基因相同。这支持了这样一种观点，即AlHV-1 C500适合开发与角马相关的MCF疫苗。
Alcelaphine herpesvirus–1 (AlHV-1), a causative agent of malignant catarrhal fever in cattle, was detected in wildebeest (Connochaetes taurinus) placenta tissue for the first time. Although viral load was low, the finding of viral DNA in over 50% of 94 samples tested lends support to the possibility that placental tissue could play a role in disease transmission and that wildebeest calves are infected in utero. Two viral loci were sequenced to examine variation among virus samples obtained from wildebeest and cattle: the ORF50 gene, encoding the lytic cycle transactivator protein, and the A9.5 gene, encoding a novel polymorphic viral glycoprotein. ORF50 was well conserved with six newly discovered alleles differing at only one or two base positions. In contrast, while only three new A9.5 alleles were discovered, these differed by up to 13% at the nucleotide level and up to 20% at the amino acid level. Structural homology searching performed with the additional A9.5 sequences determined in this study adds power to recent analysis identifying the four-helix bundle cytokine interleukin-4 (IL4) as the major homologue. The majority of MCF virus samples obtained from Tanzanian cattle and wildebeest encoded A9.5 polypeptides identical to the previously characterized A9.5 allele present in the laboratory maintained AlHV-1 C500 strain. This supports the view that AlHV-1 C500 is suitable for the development of a vaccine for wildebeest-associated MCF.
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影响因子：
2.7
作者：
ROIZMANN, B;DESROSIERS, RC;STUDDERT, MJ
通讯作者：
STUDDERT, MJ
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3.8
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作者：
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通讯作者：
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影响因子：
9.4
作者：
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