The natural product citral can cause significant damage to the hyphal cell walls of Magnaporthe grisea.
The natural product citral can cause significant damage to the hyphal cell walls of Magnaporthe grisea.
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DOI：
10.3390/molecules190710279
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发表时间：
2014-07-15
期刊：
影响因子：
--
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Li RY;Wu XM;Yin XH;Liang JN;Li M
为了寻找目前用于防治稻瘟病菌（Magnaporthe grisea）的合成杀菌剂的天然替代品，本研究探讨了柠檬醛的抗真菌潜力及其作用机制。结果表明，柠檬醛不仅能抑制菌丝的生长，而且能促进菌丝的生长。稻瘟病菌引起的菌丝形态和结构上的一系列明显变化。具体地，测试柠檬醛对M.结果表明，该化合物对稻瘟病菌的菌丝生长和菌落发育有明显的抑制作用，其IC 50和IC 90分别为40.71和203.75 μg/mL。此外，柠檬醛以浓度依赖性方式降低孢子萌发和芽管长度。在扫描电镜下观察到，柠檬醛处理后菌丝细胞表面出现褶皱和细胞破裂。有损坏的菌丝细胞壁和膜结构，损失的绒毛状物质外的细胞壁，细胞壁变薄，和不连续性形成的细胞膜处理后的基础上，透射电子显微镜（TEM）。几丁质酶活性的增加既支持菌丝中观察到的形态学变化，也表明了作用机制。总之，柠檬醛具有很强的抗真菌特性，用这种化合物处理能够对M.稻瘟病菌
In order to find a natural alternative to the synthetic fungicides currently used against the devastating rice blast fungus, Magnaporthe grisea, this study explored the antifungal potential of citral and its mechanism of action. It was found that citral not only inhibited hyphal growth of M. grisea, but also caused a series of marked hyphal morphological and structural alterations. Specifically, citral was tested for antifungal activity against M. grisea in vitro and was found to significantly inhibit colony development and mycelial growth with IC50 and IC90 values of 40.71 and 203.75 μg/mL, respectively. Furthermore, citral reduced spore germination and germ tube length in a concentration-dependent manner. Following exposure to citral, the hyphal cell surface became wrinkled with folds and cell breakage that were observed under scanning electron microscopy (SEM). There was damage to hyphal cell walls and membrane structures, loss of villous-like material outside of the cell wall, thinning of the cell wall, and discontinuities formed in the cell membrane following treatment based on transmission electron microscopy (TEM). This increase in chitinase activity both supports the morphological changes seen in the hyphae, and also suggests a mechanism of action. In conclusion, citral has strong antifungal properties, and treatment with this compound is capable of causing significant damage to the hyphal cell walls of M. grisea.
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影响因子：
3
作者：
Bolar, JP;Norelli, JL;Aldwinckle, HS
通讯作者：
Aldwinckle, HS
影响因子：
7.4
作者：
MAUCH, F;MAUCHMANI, B;BOLLER, T
通讯作者：
BOLLER, T
影响因子：
4.3
作者：
BOLLER, T;GEHRI, A;VOGELI, U
通讯作者：
VOGELI, U
影响因子：
3.3
作者：
Abbanat, D;Leighton, M;Greestein, M
通讯作者：
Greestein, M
影响因子：
7.4
作者：
SELABUURLAGE, MB;PONSTEIN, AS;CORNELISSEN, BJC
通讯作者：
CORNELISSEN, BJC