rRNA Pseudogenes in Filamentous Ascomycetes as Revealed by Genome Data.
rRNA Pseudogenes in Filamentous Ascomycetes as Revealed by Genome Data.
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基因组数据揭示丝状子囊菌中的 rRNA 假基因
DOI：
10.1534/g3.117.044016
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发表时间：
2017-08-07
期刊：
影响因子：
--
通讯作者：
Yao YJ
中科院分区：
文献类型：
--
作者：
Li Y;Yang RH;Jiang L;Hu XD;Wu ZJ;Yao YJ
核核糖体DNA（rDNA）被认为是协同进化的典范。rDNA串联重复序列（45S）广泛用于不同生物的系统发育研究，而内转录间隔区（ITS）最近被选为真菌DNA条形码。然而，rRNA假基因作为协同进化中的一种逃避机制，在许多生物中，特别是在植物和动物中被发现。此外，大量的5S rRNA假基因被确定在几个丝状子囊菌。为了研究rDNA是否以严格协调的方式进化，并测试是否存在rRNA假基因在更多种类的子囊菌，基因组内rDNA多态性进行了分析，使用全基因组序列。在检查的七种丝状子囊菌中，发现不同的rDNA旁系同源物共存于单个基因组中。通过突变分析和二级结构分析，大量旁系同源基因被鉴定为假基因。45SrDNA重复序列的三个rRNA编码区的系统发育分析，18S、5.8S和28S的同源性分析表明，这些不同的rDNA旁系同源物具有种间聚类模式。使用设计的特异性引物验证鉴定的rRNA假基因序列。突变分析表明，重复诱导的点突变（RIP）可能是负责这些rRNA假基因的形成。
The nuclear ribosomal DNA (rDNA) is considered as a paradigm of concerted evolution. Components of the rDNA tandem repeats (45S) are widely used in phylogenetic studies of different organisms and the internal transcribed spacer (ITS) region was recently selected as a fungal DNA bar code. However, rRNA pseudogenes, as one kind of escape from concerted evolution, were reported in a wide range of organisms, especially in plants and animals. Moreover, large numbers of 5S rRNA pseudogenes were identified in several filamentous ascomycetes. To study whether rDNA evolves in a strict concerted manner and test whether rRNA pseudogenes exist in more species of ascomycetes, intragenomic rDNA polymorphisms were analyzed using whole genome sequences. Divergent rDNA paralogs were found to coexist within a single genome in seven filamentous ascomycetes examined. A great number of paralogs were identified as pseudogenes according to the mutation and secondary structure analyses. Phylogenetic analyses of the three rRNA coding regions of the 45S rDNA repeats, i.e., 18S, 5.8S, and 28S, revealed an interspecies clustering pattern of those different rDNA paralogs. The identified rRNA pseudogenic sequences were validated using specific primers designed. Mutation analyses revealed that the repeat-induced point (RIP) mutation was probably responsible for the formation of those rRNA pseudogenes.
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影响因子：
64.8
作者：
INGRAM, VM
通讯作者：
INGRAM, VM
影响因子：
7
作者：
Ganley, Austen R. D.;Kobayashi, Takehiko
通讯作者：
Kobayashi, Takehiko
DOI：
10.1073/pnas.1011289108
发表时间：
2011-02-08
影响因子：
11.1
作者：
DiGuistini, Scott;Wang, Ye;Breuil, Colette
通讯作者：
Breuil, Colette
影响因子：
3
作者：
Clutterbuck, A. John
通讯作者：
Clutterbuck, A. John
影响因子：
3
作者：
Hane, James K.;Oliver, Richard P.
通讯作者：
Oliver, Richard P.