The genome sequence of Escherichia coli tailed phage D6 and the diversity of Enterobacteriales circular plasmid prophages.

The genome sequence of Escherichia coli tailed phage D6 and the diversity of Enterobacteriales circular plasmid prophages.
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DOI:
10.1016/j.virol.2017.12.019
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发表时间:
2018-03
期刊:
影响因子:
3.7
通讯作者:
Casjens SR
Casjens SR
中科院分区:
医学3区
文献类型:
--
作者:
Gilcrease EB;Casjens SR

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温和型大肠杆菌噬菌体D 6可以作为环状质粒原噬菌体存在,我们在这里报告了它的91159 bp的全基因组序列。这是一个远亲的研究噬菌体P1,但它是充分不同的,它代表了以前未描述的尾噬菌体类型或集群。对细菌基因组序列数据库的检查显示,噬菌体P1和D 6原噬菌体质粒在肠杆菌目中是常见的,此外,先前描述的沙门氏菌噬菌体SSU 5代表不同类型的温和尾噬菌体,其具有在该宿主目中也非常常见的环状质粒原噬菌体。该分析还发现了在两个较大的P1和SSU 5组(超簇)内的推定的环状质粒原噬菌体的另外的发散簇,所述两个较大的P1和SSU 5组(超簇)栖息在肠杆菌目以及γ-变形菌纲中的其他几个目的细菌中。这些序列中很少有被注释为推定的前噬菌体。
The temperate Escherichia coli bacteriophage D6 can exist as a circular plasmid prophage, and we report here its 91159 bp complete genome sequence. It is a distant relative of the well-studied phage P1, but it is sufficiently different that it typifies a previously undescribed tailed phage type or cluster. Examination of the database of bacterial genome sequences revealed that phage P1 and D6 prophage plasmids are common in the Enterobacteriales, and in addition, previously described Salmonella phage SSU5 represents a different type of temperate tailed phage with a circular plasmid prophage that is also very common in this host order. This analysis also discovered additional divergent clusters of putative circular plasmid prophages within the two larger P1 and SSU5 groups (superclusters) that inhabit the Enterobacteriales as well as bacteria in several other orders in the Gamma-proteobacteria class. Very few of these sequences are annotated as putative prophages.
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