Selenium speciation in pretreated human urine by ion-exchange chromatography and ICP-MS detection
Selenium speciation in pretreated human urine by ion-exchange chromatography and ICP-MS detection
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通过离子交换色谱和 ICP-MS 检测预处理人尿液中的硒形态
DOI：
10.1039/b008359n
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发表时间：
2001
影响因子：
3.4
通讯作者：
L. Bendahl
中科院分区：
文献类型：
--
作者：
B. Gammelgaard;O. Jøns;L. Bendahl
用苯并-15-冠-5-醚提取尿样，去除钠和钾。超过90%的钠和钾含量的尿液被这种提取去除。在pH值为3的草酸阳离子交换系统中，对未经处理的尿液池进行色谱分析，结果在前面有一个大峰和三个小峰。在冠醚处理池中至少获得了5个信号。当洗脱液为pH为3的甲酸铵时，未经处理的尿液中获得了两个小信号和一个大信号，而冠醚提取的尿液中获得了三个更明显的峰和一个前峰。在这两个体系中，两个峰与硒代蛋氨酸（SeMet）和三甲基硒鎓离子（TMSe）共洗脱。没有任何信号与硒半胱氨酸或硒代蛋氨酸共洗脱。不同个体的尿液样品中硒的浓度和比例不同。α-葡萄糖醛酸酶处理和超滤膜（截断值为10 kDa）对尿池的色谱无显著影响。用毛细管电泳ICP-MS在pH 8.2下对尿池进行分析，可分离出4个峰。其中一个峰值与SeMet共迁移，而TMSe未在该系统中出现。冠醚萃取对分离效果无显著影响。因此，除了SeMet和TMSe，至少还有三种未知的含硒物质存在于尿液中。
Urine samples were extracted by benzo-15-crown-5-ether to remove sodium and potassium. More than 90% of the sodium and potassium content of the urine was removed with this extraction. In a cation-exchange system based on oxalic acid at pH 3, chromatography of an untreated urine pool resulted in a large peak in the front together with three small peaks. In the crown ether treated pool at least five signals were obtained. When the eluent was ammonium formate at pH 3, two small signals together with a large signal in the front were obtained in untreated urine, while three more distinct peaks and a peak in front were obtained in the crown ether extracted urine. In both systems, two of the peaks co-eluted with selenomethionine (SeMet) and the trimethylselenonium ion (TMSe). None of the signals co-eluted with either selenocystine or selenoethionine. Urine samples from different individuals showed different concentrations and ratios of the selenium species present. There was no difference in the chromatograms when the urine pool was treated with α-glucuronidase or ultrafiltrated through a membrane with a cut-off value of 10 kDa. When the urine pool was analysed by capillary electrophoresis ICP-MS at pH 8.2, four peaks could be separated. One of the peaks co-migrated with SeMet while TMSe did not appear in this system. Crown ether extraction did not improve the separation. Hence, apart from SeMet and TMSe, at least three more unknown selenium-containing species were present in urine.
三甲基硒离子在人尿中的排泄。
影响因子：
2.9
作者：
Sun,XF;Ting,BT;Janghorbani,M
通讯作者：
Janghorbani,M
由[75Se]硒代胱氨酸合成[75Se]三甲基碘化硒。
影响因子：
2.9
作者：
Foster,SJ;Ganther,HE
通讯作者：
Ganther,HE