Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress.
Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress.
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DOI：
10.1186/s12870-021-03339-1
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发表时间：
2021-12-09
影响因子：
5.3
通讯作者：
Zhou M
中科院分区：
文献类型：
--
作者：
Papolu PK;Ramakrishnan M;Wei Q;Vinod KK;Zou LH;Yrjala K;Kalendar R;Zhou M
LTR反转录转座子在植物生长、基因组进化和环境胁迫反应中起着重要作用，但其对热胁迫的调控作用尚不清楚。研究了毛竹（Phyllostachys edulis）两个LTR反转录转座子PHRE 1和PHRE 2在高温胁迫下的活性变化。PHRE 1和PHRE 2在有或没有CaMV 35 s启动子的情况下的差异过表达在热胁迫下在转基因植物中表现出增强的表达。转录活性研究表明，热胁迫下转基因毛竹和拟南芥转基因植株的转座活性和拷贝数均有所增加。转基因植株中启动子活性比较表明，5 'LTR启动子活性高于CaMV 35 s启动子。此外，酵母单杂交（Y1 H）系统和植物生物分子荧光互补（BiFC）分析揭示了热依赖性转录因子（TF）与5 'LTR序列的相互作用以及TF与pol和gag的直接相互作用。以上结果表明，5 'LTR在高温胁迫下作为启动子对毛竹LTR反转录转座子的表达起调控作用。在线版本包含补充材料，可通过10.1186/s12870-021-03339-1获得。
LTR retrotransposons play a significant role in plant growth, genome evolution, and environmental stress response, but their regulatory response to heat stress remains unclear. We have investigated the activities of two LTR retrotransposons, PHRE1 and PHRE2, of moso bamboo (Phyllostachys edulis) in response to heat stress. The differential overexpression of PHRE1 and PHRE2 with or without CaMV35s promoter showed enhanced expression under heat stress in transgenic plants. The transcriptional activity studies showed an increase in transposition activity and copy number among moso bamboo wild type and Arabidopsis transgenic plants under heat stress. Comparison of promoter activity in transgenic plants indicated that 5’LTR promoter activity was higher than CaMV35s promoter. Additionally, yeast one-hybrid (Y1H) system and in planta biomolecular fluorescence complementation (BiFC) assay revealed interactions of heat-dependent transcription factors (TFs) with 5’LTR sequence and direct interactions of TFs with pol and gag. Our results conclude that the 5’LTR acts as a promoter and could regulate the LTR retrotransposons in moso bamboo under heat stress. The online version contains supplementary material available at 10.1186/s12870-021-03339-1.
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影响因子：
14.9
作者：
Goffin, W;Demonté, D;Van Lint, C
通讯作者：
Van Lint, C
GATA转录因子GNC在杨树的光合作用和生长中发挥着重要作用
影响因子：
6.9
作者：
An, Yi;Zhou, Yangyan;Xia, Xinli
通讯作者：
Xia, Xinli
DOI：
10.1016/j.bbagrm.2014.07.017
发表时间：
2015-04-01
影响因子：
4.7
作者：
Grandbastien, Marie-Angele
通讯作者：
Grandbastien, Marie-Angele
DOI：
10.1073/pnas.1903936116
发表时间：
2019-09-03
影响因子：
11.1
作者：
Cappucci, Ugo;Noro, Fabrizia;Pimpinelli, Sergio
通讯作者：
Pimpinelli, Sergio
影响因子：
8.3
作者：
De Felice B;Wilson RR;Argenziano C;Kafantaris I;Conicella C
通讯作者：
Conicella C