A transcriptionally active copia-like retroelement in Citrus limon.
A transcriptionally active copia-like retroelement in Citrus limon.
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DOI：
10.2478/s11658-008-0050-5
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发表时间：
2009
影响因子：
8.3
通讯作者：
Conicella C
中科院分区：
文献类型：
--
作者：
De Felice B;Wilson RR;Argenziano C;Kafantaris I;Conicella C
植物核基因组主要由可移动的DNA组成，它可以重排基因组和其他单个基因的结构，并通过各种促进活动影响基因的调控：转座、插入、切除、染色体断裂和异位重组。Ty 1-copia类反转录转座子是植物界广泛存在的一类转座因子，占总DNA含量的很大一部分。在这里，一种新的逆转录转座子样序列被分离和鉴定为Ty 1-copia样逆转录酶结构域（命名为CLCoy 1），基于已知元件的同源性。荧光原位杂交结果表明，CLCoy 1主要定位于柑橘染色体的端粒和亚端粒区域，分布于染色体的沿着。CLCoy 1占基因组的3.6%，有趣的是，虽然转座子大多数是特定于一个物种，但该元件在其他柑橘属物种中被鉴定出，如柑橘，玛格丽塔和柑橘，但在枳中未检测到。我们还确定，创伤，盐和细胞培养胁迫产生的转录激活这种新的逆转录元件在柑橘利蒙。新的Ty 1 copia样元件CLCoy 1可能发挥了重要作用，在柑橘物种进化过程中塑造基因组结构和大小。
The plant nuclear genome is largely composed of mobile DNA, which can rearrange genomes and other individual gene structure and also affect gene regulation through various promoted activities: transposition, insertion, excision, chromosome breakage, and ectopic recombination. Ty1-copia-like retrotransposon is a widespread class of transposable elements in the plant kingdom, representing a large part of the total DNA content. Here, a novel retrotransposon-like sequence was isolated and identified as the Ty1-copia-like reverse transcriptase domain (named here CLCoy1), based on the homology of known elements. Fluorescence in situ hybridization, revealed that CLCoy1 was mainly located in telomeric and sub-telomeric regions along the Citrus chromosomes. CLCoy1 composes 3.6% of the genome and, interestingly, while transposons are mostly specific to a species, this element was identified in other Citrus species such as Citrus aurantium, Fortunella margarita and Citrus paradisi, but undetected in Poncirus trifoliata. We also determined that wounding, salt and cell culture stress produced transcriptional activation of this novel retroelement in Citrus limon. The novel Ty1-copia-like element CLCoy1 may have played a major role in shaping genome structure and size during Citrus species evolution.
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DOI：
10.1073/pnas.93.15.7783
发表时间：
1996-07-23
影响因子：
11.1
作者：
Hirochika, H;Sugimoto, K;Kanda, M
通讯作者：
Kanda, M
DOI：
10.1073/pnas.97.13.7002
发表时间：
2000-06-20
影响因子：
11.1
作者：
Fedoroff, N
通讯作者：
Fedoroff, N
DOI：
10.1073/pnas.89.9.4018
发表时间：
1992-05-01
影响因子：
11.1
作者：
MILLER, WJ;HAGEMANN, S;PINSKER, W
通讯作者：
PINSKER, W
影响因子：
7.2
作者：
Suoniemi, A;Tanskanen, J;Schulman, AH
通讯作者：
Schulman, AH
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M