Ultracytochemical demonstration of ATP-dependent calcium pump in ameloblasts of rat incisor enamel organ.
Ultracytochemical demonstration of ATP-dependent calcium pump in ameloblasts of rat incisor enamel organ.
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大鼠门牙釉质器官成釉细胞中 ATP 依赖性钙泵的超细胞化学演示。
DOI：
10.1007/bf02553296
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发表时间：
1986
影响因子：
4.2
通讯作者：
Garant,PR
中科院分区：
文献类型：
--
作者：
Sasaki,T;Garant,PR
将生长中的大鼠门牙的牙釉质器官用甲醛和戊二醛的混合物固定，并通过一步引导技术在碱性 pH 条件下进行处理，以进行 Ca 和 Mg 激活的膜 ATP 酶的超细胞化学演示。为了抑制非特异性碱性磷酸酶，将 5 mM 左旋咪唑添加到孵育介质中。除了近端和远端连接复合物以及侧面和基底细胞表面的间隙连接外，分泌性成釉细胞的细胞表面显示出强烈的 Ca-和 Mg-ATP 酶活性。面向棒间牙釉质生长区域和棒状牙釉质生长区域的 Tomes 突近端和远端部分的深层质膜内陷表现出最强的酶反应。 Mg-ATP酶活性也被证明存在于分泌性成釉细胞的质膜中，但其强度不如Ca-ATP酶。除高尔基膜发生轻微反应外，分泌性成釉细胞和邻近釉质基质的所有其他细胞器均未发生酶促反应。然而，当将组织在缺乏左旋咪唑的培养基中孵育时，在杆和杆间生长区域的新分泌的牙釉质基质以及细胞的质膜上观察到显着的酶反应。在皱边型和平滑端型的成熟成釉细胞中，Ca-和Mg-ATP酶的弱反应仅限于面向乳头细胞层的基底细胞表面。在缺乏左旋咪唑的培养基中培养的组织中，在高尔基体膜、线粒体膜、皱褶边界区的平滑内质网的管状元件以及沿着皱褶边界的质膜中观察到反应产物的不同沉积。在整个分泌和成熟阶段，当在含有左旋咪唑的培养基中孵育时，在中间层和乳头层细胞的质膜中观察到Ca-和Mg-ATP酶的中度和/或弱酶促反应。 Ca-ATP酶培养介质中省略底物ATP和/或酶激活剂CaCl2会在检查的组织中产生阴性反应。当钙调蛋白阻滞剂三氟拉嗪静脉注射给大鼠时，分泌性成釉细胞质膜上的 Ca-ATP 酶活性几乎完全消失，但其他细胞类型的质膜却没有。
The enamel organ of the growing rat incisor was fixed with a mixture of formaldehyde and glutaraldehyde and processed for ultracytochemical demonstration of Ca- and Mg-activated membrane ATPase by a one-step lead technique at alkaline pH. To inhibit nonspecific alkaline phosphatase, 5 mM levamisole was added to the incubation media. Intense Ca- and Mg-ATPase activity was demonstrated in the cell surfaces of the secretory ameloblasts, except at the proximal and distal junctional complexes and the gap junctions in the lateral and basal cell surfaces. Deep plasma membrane invaginations at the proximal and distal parts of Tomes processes facing interrod- and rod-enamel growth regions exhibited the strongest enzymatic reaction. Mg-ATPase activity was also shown to be present in the plasma membranes of secretory ameloblasts but it was less intense than Ca-ATPase. Except for a slight reaction in the Golgi membranes, all other cell organelles of the secretory ameloblasts and the adjacent enamel matrix were free of enzymatic reaction. However, when the tissues were incubated in media lacking levamisole, a prominent enzymatic reaction was observed in the newly secreted enamel matrix of the rod and interrod growth regions as well as on the plasma membranes of the cells. In maturation ameloblasts of both ruffleended and smooth-ended types, a weak reaction for Ca- and Mg-ATPase was restricted to basal cell surfaces facing the papillary cell layer. In tissues incubated in media lacking levamisole, a variable deposition of reaction products was observed in the Golgi membranes, mitochondrial membranes, tubular elements of smooth endoplasmic reticulum in the ruffled border zone, and along the plasma membranes of the ruffled border. Throughout the secretory and maturation stages, a moderate and/or weak enzymatic reaction for both Ca- and Mg-ATPase was seen in the plasma membranes of the cells of the stratum intermedium and the papillary layer when incubated in media with levamisole. Omission of substrate ATP and/or the enzyme activator CaCl2from the incubation media for Ca-ATPase produced a negative reaction in the tissues examined. When the calmodulin blocker trifluoperazine was administered to the rats intravenously, Ca-ATPase activity was almost completely abolished from the plasma membranes of secretory ameloblasts, but not of other cell types.
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影响因子：
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DOI：
10.1016/0277-5379(83)90405-4
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期刊：
European journal of cancer & clinical oncology
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影响因子：
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影响因子：
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作者：
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DOI：
10.1111/j.1432-1033.1981.tb06218.x
发表时间：
1981-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
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作者：
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通讯作者：
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