Neurological scoring and gait kinematics to assess functional outcome in an ovine model of ischaemic stroke.
Neurological scoring and gait kinematics to assess functional outcome in an ovine model of ischaemic stroke.
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DOI：
10.3389/fneur.2023.1071794
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发表时间：
2023
影响因子：
3.4
通讯作者：
Jones, Claire F.
中科院分区：
文献类型：
--
作者：
Sorby-Adams, Annabel J.;Marian, Oana C.;Bilecki, Isabella M.;Elms, Levi E.;Camargo, Jonathan;Hall, Kelly;Crowther, Robert G.;Leonard, Anna V.;Wadsworth, George I.;Spear, Joshua H.;Turner, Renee J.;Jones, Claire F.
评估缺血性脑卒中后的功能损害对于确定临床患者和临床前模型的干预结果和疗效至关重要。虽然啮齿类动物的范例已经得到了很好的描述，但用于大型动物（如绵羊）的可比方法仍然有限。本研究旨在利用运动捕捉的复合神经学评分和步态运动学来评估绵羊缺血性中风模型的功能。将26只美利奴羊麻醉，阻断大脑中动脉2小时。动物在基线（中风前8天、5天和1天）和中风后3天进行功能评估。进行神经评分以确定神经状态的变化。10台红外摄像机测量了42个反向反射标记的轨迹，用于计算步态运动学。脑卒中后3天进行磁共振成像（MRI）以确定梗死面积。使用类内相关系数（ICC）来评估基线试验中神经评分和步态运动学的可重复性。所有基线的平均值用于比较中风后3天神经评分和运动学的变化。采用主成分分析（PCA）确定脑卒中后神经评分、步态运动学和梗死体积之间的关系。神经系统评分在基线试验中具有中等可重复性（ICC > 0.50），并在脑卒中后检测到明显的损伤（p < 0.05）。基线步态测量对大多数评估变量显示中等至良好的可重复性（ICC > 0.50）。卒中后，运动学指标显示卒中缺陷，包括站立和步幅持续时间的增加（p < 0.05）。MRI显示梗死累及皮质和/或丘脑（中位数2.7 cm3, IQR 1.4至11.9）。PCA产生了两个成分，尽管变量之间的关联不确定。这项研究开发了可重复的方法来评估羊的功能，使用复合评分和步态运动学，允许评估中风后3天的缺陷。尽管每种方法都独立使用，但在PCA上观察到步态运动学、综合评分和梗死体积之间的相关性很差。这表明，这些措施中的每一个都对卒中缺陷的评估有谨慎的效用，并且多模式方法对于全面表征功能损伤是必要的。
Assessment of functional impairment following ischaemic stroke is essential to determine outcome and efficacy of intervention in both clinical patients and pre-clinical models. Although paradigms are well described for rodents, comparable methods for large animals, such as sheep, remain limited. This study aimed to develop methods to assess function in an ovine model of ischaemic stroke using composite neurological scoring and gait kinematics from motion capture. Merino sheep (n = 26) were anaesthetised and subjected to 2 hours middle cerebral artery occlusion. Animals underwent functional assessment at baseline (8-, 5-, and 1-day pre-stroke), and 3 days post-stroke. Neurological scoring was carried out to determine changes in neurological status. Ten infrared cameras measured the trajectories of 42 retro-reflective markers for calculation of gait kinematics. Magnetic resonance imaging (MRI) was performed at 3 days post-stroke to determine infarct volume. Intraclass Correlation Coefficients (ICC's) were used to assess the repeatability of neurological scoring and gait kinematics across baseline trials. The average of all baselines was used to compare changes in neurological scoring and kinematics at 3 days post-stroke. A principal component analysis (PCA) was performed to determine the relationship between neurological score, gait kinematics, and infarct volume post-stroke. Neurological scoring was moderately repeatable across baseline trials (ICC > 0.50) and detected marked impairment post-stroke (p < 0.05). Baseline gait measures showed moderate to good repeatability for the majority of assessed variables (ICC > 0.50). Following stroke, kinematic measures indicative of stroke deficit were detected including an increase in stance and stride duration (p < 0.05). MRI demonstrated infarction involving the cortex and/or thalamus (median 2.7 cm3, IQR 1.4 to 11.9). PCA produced two components, although association between variables was inconclusive. This study developed repeatable methods to assess function in sheep using composite scoring and gait kinematics, allowing for the evaluation of deficit 3 days post-stroke. Despite utility of each method independently, there was poor association observed between gait kinematics, composite scoring, and infarct volume on PCA. This suggests that each of these measures has discreet utility for the assessment of stroke deficit, and that multimodal approaches are necessary to comprehensively characterise functional impairment.
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影响因子：
4.6
作者：
Broom L;Ellison BA;Worley A;Wagenaar L;Sörberg E;Ashton C;Bennett DA;Buchman AS;Saper CB;Shih LC;Hausdorff JM;VanderHorst VG
通讯作者：
VanderHorst VG
血管认知障碍的翻译模型：包括较大物种的综述。
影响因子：
9.3
作者：
Hainsworth AH;Allan SM;Boltze J;Cunningham C;Farris C;Head E;Ihara M;Isaacs JD;Kalaria RN;Lesnik Oberstein SA;Moss MB;Nitzsche B;Rosenberg GA;Rutten JW;Salkovic-Petrisic M;Troen AM
通讯作者：
Troen AM
影响因子：
8.3
作者：
Fisher M;Feuerstein G;Howells DW;Hurn PD;Kent TA;Savitz SI;Lo EH;STAIR Group
通讯作者：
STAIR Group
DOI：
10.1002/ar.22955
发表时间：
2014-10
期刊：
Anatomical record (Hoboken, N.J. : 2007)
影响因子：
--
作者：
Batka RJ;Brown TJ;Mcmillan KP;Meadows RM;Jones KJ;Haulcomb MM
通讯作者：
Haulcomb MM
影响因子：
1.8
作者：
Brouwer, Brenda;Parvataneni, Krishnaji;Olney, Sandra J.
通讯作者：
Olney, Sandra J.