Enterobacter sp. Strain SM1_HS2B Manifests Transient Elongation and Swimming Motility in Liquid Medium.
Enterobacter sp. Strain SM1_HS2B Manifests Transient Elongation and Swimming Motility in Liquid Medium.
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DOI：
10.1128/spectrum.02078-21
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发表时间：
2022-06-29
影响因子：
3.7
通讯作者：
Tang, Jay X.
中科院分区：
文献类型：
--
作者：
Zhang, Zhiyu;Liu, Haoming;Karani, Hamid;Mallen, Jon;Chen, Weijie;De, Arpan;Mani, Sridhar;Tang, Jay X.
关键词：
许多种类的细菌在外部压力下会改变其形态和行为。在这项研究中，我们报告了一种新型肠杆菌属的瞬时伸长和游泳运动。菌株 SM1_HS2B，在标准生长条件下的液体肉汤中。当在 Luria-Bertani 培养基中生长时，HS2B 细胞会延迟细胞分裂并伸长。虽然在几个小时内是短暂的，但平均细胞长度达到静止状态细胞的 10 倍以上。通过将细胞从指数期取出并每 2 小时添加到新鲜培养基中刺激重复的生长周期，这种增加也会累积。大多数细胞在指数生长期获得游泳活力，然后在几个小时内失去游泳活力。由于长游泳细胞的分裂，两个子细胞都保留了游泳的能力。我们确认长 HS2B 细胞沿着其身体轴以刚体旋转的方式游动。这些发现基于重复生长周期后的显微镜观察，将 HS2B 确立为原型菌株，其大小和运动性对其物理和生化环境具有敏感依赖性。重要性 细菌会发生形态变化以应对外部压力。最著名的例子是集群运动背景下的细胞伸长和过度鞭毛。本报告的主题 SM1_HS2B 是一种新鉴定的肠杆菌种（称为肠杆菌属 sp.）的超群菌株。 SM1。 SM1_HS2B 在新鲜液体培养基中短暂伸长至极端长度的关键发现为细菌生长和分裂的调节提供了新的见解。 SM1_HS2B 在液体培养基中的指数生长阶段也表现出短暂但剧烈的游泳运动。这些特性使 HS2B 成为一种原型菌株，其大小和运动性对其物理和生化环境具有敏感依赖性。这种依赖性可能与具有显着环境或生理结果的集群行为有关。
Many species of bacteria change their morphology and behavior under external stresses. In this study, we report transient elongation and swimming motility of a novel Enterobacter sp. strain, SM1_HS2B, in liquid broth under a standard growth condition. When growing in the Luria-Bertani medium, HS2B cells delay their cell division and elongate. Although transient over a few hours, the average cell length reaches over 10 times that of the stationary-state cells. The increase is also cumulative following repeated growth cycles stimulated by taking cells out of the exponential phase and adding them into fresh medium every 2 hours. The majority of the cells attain swimming motility during the exponential growth phase, and then they lose swimming motility over the course of several hours. Both daughter cells due to division of a long swimming cell retain the ability to swim. We confirm that the long HS2B cells swim with rigid-body rotation along their body axis. These findings based on microscopic observation following repeated cycles of growth establish HS2B as a prototype strain with sensitive dependence of size and motility on its physical and biochemical environment. IMPORTANCE Bacteria undergo morphological changes in order to cope with external stresses. Among the best-known examples are cell elongation and hyperflagellation in the context of swarming motility. The subject of this report, SM1_HS2B, is a hyperswarming strain of a newly identified species of enterobacteria, noted as Enterobacter sp. SM1. The key finding that SM1_HS2B transiently elongates to extreme length in fresh liquid medium offers new insights on regulation in bacterial growth and division. SM1_HS2B also manifests transient but vigorous swimming motility during the exponential phase of growth in liquid medium. These properties establish HS2B as a prototype strain with sensitive dependence of size and motility on its physical and biochemical environment. Such a dependence may be relevant to swarming behavior with a significant environmental or physiological outcome.
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影响因子：
29.4
作者：
De A;Chen W;Li H;Wright JR;Lamendella R;Lukin DJ;Szymczak WA;Sun K;Kelly L;Ghosh S;Kearns DB;He Z;Jobin C;Luo X;Byju A;Chatterjee S;San Yeoh B;Vijay-Kumar M;Tang JX;Prajapati M;Bartnikas TB;Mani S
通讯作者：
Mani S
DOI：
10.1016/j.ijfoodmicro.2006.04.026
发表时间：
2006-12-01
影响因子：
5.4
作者：
Hazeleger, Wilma C.;Dalvoorde, Marinka;Beumer, Rijkelt R.
通讯作者：
Beumer, Rijkelt R.
影响因子：
4.4
作者：
Kumar, Govindaraj Dev;Macarisin, Dumitru;Micallef, Shirley A.
通讯作者：
Micallef, Shirley A.
影响因子：
2.4
作者：
Wainwright, M;Canham, LT;Reeves, CL
通讯作者：
Reeves, CL
影响因子：
4.6
作者：
Partridge JD;Ariel G;Schvartz O;Harshey RM;Be'er A
通讯作者：
Be'er A