Ectoine production by Alkalibacillus haloalkaliphilus—Bioprocess development using response surface methodology and model‐driven strategies
Ectoine production by Alkalibacillus haloalkaliphilus—Bioprocess development using response surface methodology and model‐driven strategies
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通过嗜碱碱杆菌生产四氢嘧啶-使用响应面方法和模型驱动策略进行生物工艺开发
DOI：
10.1002/elsc.201200151
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发表时间：
2013
影响因子：
2.7
通讯作者：
Krull R.
中科院分区：
文献类型：
--
作者：
Bergmann S;David F;Franco-Lara E;Wittmann C;Krull R.
嗜盐微生物是重要的碱生产者，在工业上得到了广泛的应用。生长和渗透调节是根据环境分布的多样性来区分的，需要对栽培条件和渗透反应进行全面的研究。在环境胁迫条件下生长的嗜盐细菌——嗜盐嗜碱杆菌能够积累相容的溶质异托碱。由于微生物生长和产异构体是工艺关键参数（如盐度、pH值和温度）的函数，因此优化产生了三种重要的工艺策略，并通过响应面法进行了估计。对于每种策略，使用新开发的染色方法进行单细胞分析，直接在培养基中监测嗜盐微生物的膜电位。流式细胞术可以揭示异托因生产过程中的生理状态，在基础研究、工艺优化和质量控制等方面具有很大的应用潜力。
Halophile microorganisms are important ectoine producers and are applied in industrial applications. The growth and osmoregulation are distinguished based on the diversity of environmental distribution and require holistic investigations of cultivation condition and osmotic response. The barely investigated halophilic bacteriumAlkalibacillus haloalkaliphilusis able to accumulate the compatible solute ectoine, while growing under environmental stress conditions. Since microbial growth and ectoine production are functions of the process key parameters such as salinity, pH value, and temperature, the optimization resulted in three significant process strategies that were estimated by response surface methodology. For each strategy, single‐cell analysis was performed using a newly developed staining method for monitoring the membrane potential of halophilic microorganisms directly in the cultivation medium. The flow cytometric method was shown to reveal the physiological state during ectoine production and has a great potential to be applied for basic research, process optimization, and quality control.
影响因子：
3.4
作者：
WEISSER, J;TRUPER, HG
通讯作者：
TRUPER, HG
通过响应面法优化生长培养基，以获得嗜盐古菌（盐杆菌科）的高细胞密度培养物。
影响因子：
11.4
作者：
M. Manikandan;L. Pasic;V. Kannan
通讯作者：
V. Kannan