Identification of natural compounds with antiviral activities against SARS-associated coronavirus.
Identification of natural compounds with antiviral activities against SARS-associated coronavirus.
复制标题
DOI：
10.1016/j.antiviral.2005.02.007
复制
发表时间：
2005-07
影响因子：
7.6
通讯作者：
Tan X
中科院分区：
文献类型：
--
作者：
Li SY;Chen C;Zhang HQ;Guo HY;Wang H;Wang L;Zhang X;Hua SN;Yu J;Xiao PG;Li RS;Tan X
采用3-（4，5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺基苯基）-2H-四氮唑内盐（MTS）法对200多种中草药提取物进行抗SARS冠状病毒活性筛选。其中4种提取物对SARS-CoV具有中等至强的抗病毒活性，半数有效浓度（EC 50）为2.4 ± 0.2 ~ 88.2 ± 7.7 μg/ml。四种植物中，石蒜的药效最强。为了识别活性组分，L.辐射提取物进行进一步的分级分离、纯化和CPE/MTS测定。该过程导致鉴定出单一物质石蒜碱作为抗SARS-CoV组分，其EC 50值为15.7 ± 1.2 nM。该化合物在细胞毒性试验中的CC 50值为14980.0 ± 912.0 nM，选择性指数（SI）大于900。结果表明，4种中药提取物和复方石蒜碱有可能成为抗SARS病毒新药的候选药物。
More than 200 Chinese medicinal herb extracts were screened for antiviral activities against Severe Acute Respiratory Syndrome-associated coronavirus (SARS-CoV) using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay for virus-induced cytopathic effect (CPE). Four of these extracts showed moderate to potent antiviral activities against SARS-CoV with 50% effective concentration (EC50) ranging from 2.4 ± 0.2 to 88.2 ± 7.7 μg/ml. Out of the four, Lycoris radiata was most potent. To identify the active component, L. radiata extract was subjected to further fractionation, purification, and CPE/MTS assays. This process led to the identification of a single substance lycorine as an anti-SARS-CoV component with an EC50 value of 15.7 ± 1.2 nM. This compound has a CC50 value of 14980.0 ± 912.0 nM in cytotoxicity assay and a selective index (SI) greater than 900. The results suggested that four herbal extracts and the compound lycorine are candidates for the development of new anti-SARS-CoV drugs in the treatment of SARS.
登录
查看更多内容
DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
影响因子：
16.2
作者：
CORY, AH;OWEN, TC;CORY, JG
通讯作者：
CORY, JG
影响因子：
5.4
作者：
VLIETINCK, AJ;VANDENBERGHE, DA
通讯作者：
VANDENBERGHE, DA
影响因子：
5.4
作者：
McCutcheon, AR;Roberts, TE;Towers, GHN
通讯作者：
Towers, GHN
影响因子：
3.8
作者：
ALI, AA;KATING, H;RAMADAN, MA
通讯作者：
RAMADAN, MA