Genetic diversity in the block 2 region of the merozoite surface protein-1 of Plasmodium falciparum in central India.
Genetic diversity in the block 2 region of the merozoite surface protein-1 of Plasmodium falciparum in central India.
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DOI：
10.1186/1475-2875-11-78
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发表时间：
2012-03-22
期刊：
影响因子：
3
通讯作者：
Singh N
中科院分区：
文献类型：
--
作者：
Bharti PK;Shukla MM;Sharma YD;Singh N
疟疾仍然是印度的一个重大健康问题。几种预期的恶性疟原虫疫苗候选抗原是高度多态性的。恶性疟原虫裂殖子表面蛋白-1（MSP-1）的遗传多样性已在世界各地得到广泛研究。然而，印度提供的数据有限。本研究的目的是MSP-1基因的块2区域的分子特征，从部落为主，森林地区的中央邦。对2005年7月至2005年11月收集的71株田间分离株和2009年7月至2009年12月收集的98株田间分离株进行了DNA测序分析。DNA测序鉴定的等位基因与3D 7株进行比对，并利用DNASTAR软件进行多态性分析。2005年疟疾阳性率为26%，2009年上升到29%，恶性疟原虫流行率也从2005年的72%上升到2009年的81%。2005年，K1（51%）的总体等位基因患病率较高，其次是MAD 20（28%）和RO 33（21%），而在2009年，RO 33最高（40%），其次是K1（36%）和MAD 20（24%）。本研究报告了广泛的遗传变异和动态进化的MSP-1在印度中部的块2区。疫苗候选抗原的抗原多样性的表征对于未来的疫苗试验以及了解该地区恶性疟原虫寄生虫的种群动态是有价值的。
Malaria continues to be a significant health problem in India. Several of the intended Plasmodium falciparum vaccine candidate antigens are highly polymorphic. The genetic diversity of P. falciparum merozoite surface protein-1 (MSP-1) has been extensively studied from various parts of the world. However, limited data are available from India. The aim of the present study was a molecular characterization of block 2 region of MSP-1 gene from the tribal-dominated, forested region of Madhya Pradesh. DNA sequencing analysis was carried out in 71 field isolates collected between July 2005 to November 2005 and in 98 field isolates collected from July 2009 to December 2009. Alleles identified by DNA sequencing were aligned with the strain 3D7 and polymorphism analysis was done by using Edit Sequence tool (DNASTAR). The malaria positivity was 26% in 2005, which rose to 29% in 2009 and P. falciparum prevalence was also increased from 72% in 2005 to 81% in 2009. The overall allelic prevalence was higher in K1 (51%) followed by MAD20 (28%) and RO33 (21%) in 2005 while in 2009, RO33 was highest (40%) followed by K1 (36%) and MAD20 (24%). The present study reports extensive genetic variations and dynamic evolution of block 2 region of MSP-1 in central India. Characterization of antigenic diversity in vaccine candidate antigens are valuable for future vaccine trials as well as understanding the population dynamics of P. falciparum parasites in this area.
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