Comparing laser speckle contrast imaging and indocyanine green angiography for assessment of parathyroid perfusion.
Comparing laser speckle contrast imaging and indocyanine green angiography for assessment of parathyroid perfusion.
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DOI：
10.1038/s41598-023-42649-2
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发表时间：
2023-10-12
影响因子：
4.6
通讯作者：
Mahadevan-Jansen, Anita
中科院分区：
文献类型：
--
作者：
Mannoh, Emmanuel A.;Baregamian, Naira;Thomas, Giju;Solorzano, Carmen C.;Mahadevan-Jansen, Anita
术中准确评估甲状旁腺血流对术后功能保护至关重要。吲哚菁绿色（ICG）血管造影术已成功应用，但其传统应用有局限性。无标记方法克服了这些限制，激光散斑对比成像（LSCI）就是这样一种方法，可以准确地检测和量化甲状旁腺灌注的差异。在这项研究中，招募了21名接受甲状腺切除术或甲状旁腺切除术的患者，以比较术中LSCI和ICG荧光。使用实验成像设备对总共37个甲状旁腺进行成像。由三名观察者基于感知强度为ICG荧光分配0、1或2分：0分表示几乎没有荧光，1分表示中等或斑片状荧光，2分表示强荧光。根据这些分数对斑点对比度值进行分组。进行方差分析以检测组间的显著差异。最后，计算每个甲状旁腺的ICG荧光强度，并在线性回归中与散斑对比度进行比较。结果显示组间斑点对比度存在显著差异，例如ICG评分为0的甲状旁腺的斑点对比度高于ICG评分为1的甲状旁腺，而ICG评分为1的甲状旁腺的斑点对比度又高于ICG评分为2的甲状旁腺。平均归一化ICG荧光强度和斑点对比度之间的相关系数为-0.81进一步支持了这一点。这表明ICG血管造影术和LSCI检测到甲状旁腺血流的相似差异。激光散斑对比成像显示出作为无标记替代方案的前景，克服了ICG血管造影术用于甲状旁腺评估的当前局限性。
Accurate intraoperative assessment of parathyroid blood flow is crucial to preserve function postoperatively. Indocyanine green (ICG) angiography has been successfully employed, however its conventional application has limitations. A label-free method overcomes these limitations, and laser speckle contrast imaging (LSCI) is one such method that can accurately detect and quantify differences in parathyroid perfusion. In this study, twenty-one patients undergoing thyroidectomy or parathyroidectomy were recruited to compare LSCI and ICG fluorescence intraoperatively. An experimental imaging device was used to image a total of 37 parathyroid glands. Scores of 0, 1 or 2 were assigned for ICG fluorescence by three observers based on perceived intensity: 0 for little to no fluorescence, 1 for moderate or patchy fluorescence, and 2 for strong fluorescence. Speckle contrast values were grouped according to these scores. Analyses of variance were performed to detect significant differences between groups. Lastly, ICG fluorescence intensity was calculated for each parathyroid gland and compared with speckle contrast in a linear regression. Results showed significant differences in speckle contrast between groups such that parathyroids with ICG score 0 had higher speckle contrast than those assigned ICG score 1, which in turn had higher speckle contrast than those assigned ICG score 2. This was further supported by a correlation coefficient of -0.81 between mean-normalized ICG fluorescence intensity and speckle contrast. This suggests that ICG angiography and LSCI detect similar differences in blood flow to parathyroid glands. Laser speckle contrast imaging shows promise as a label-free alternative that overcomes current limitations of ICG angiography for parathyroid assessment.
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DOI：
10.1007/0-387-27530-4_1
发表时间：
2005-01-01
期刊：
MOLECULAR BIOLOGY OF THE PARATHYROID
影响因子：
--
作者：
Günther, T;Karsenty, G
通讯作者：
Karsenty, G
影响因子：
5
作者：
Johnson, Nathan A.;Tublin, Mitchell E.;Ogilvie, Jennifer B.
通讯作者：
Ogilvie, Jennifer B.
影响因子：
2.5
作者：
Kim, Yoo Seok;Erten, Ozgun;Berber, Eren
通讯作者：
Berber, Eren
影响因子：
--
作者：
Lo, CY;Tam, SC
通讯作者：
Tam, SC
影响因子：
6.6
作者：
Mannoh, Emmanuel A.;Thomas, Giju;Mahadevan-Jansen, Anita
通讯作者：
Mahadevan-Jansen, Anita