Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model.
Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model.
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脂肪干细胞接种的膀胱脱细胞基质移植丝素蛋白增强大鼠模型中的膀胱重建
DOI：
10.18632/oncotarget.21211
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发表时间：
2017-10-17
期刊：
影响因子：
--
通讯作者：
Lu M
中科院分区：
文献类型：
--
作者：
Xiao D;Wang Q;Yan H;Lv X;Zhao Y;Zhou Z;Zhang M;Sun Q;Sun K;Li W;Lu M
寄主细胞植入膀胱增强支架的临床效果不佳，需要改进生物活性生物材料。本研究旨在探讨脂肪源性干细胞(ASCs)-种子双层膀胱脱细胞基质移植(BAMG)-丝素蛋白（SF）支架促进膀胱重建的可行性。将Sprague - Dawley大鼠随机分为3组：BAMG-SF- ascs组、脱细胞BAMG-SF组和膀胱切除术组。BAMG-SF-ASCs组在2周、4周和12周时取样，并与其他组在12周时进行比较。在BAMG-SF-ASCs组中，膀胱轮廓与膀胱切开术组相似，尿路上皮和平滑肌再生丰富，支架降解速度适宜，纤维化和炎症轻微。在12周的实验期间，BAMG-SF中植入的ASCs维持在再生区域，与无细胞BAMG-SF相比，血管密度、神经再生和膀胱功能显著增强。此外，BAMG-SF-ASCs组SDF-1α、VEGF及其受体水平升高，ERK 1/2磷酸化水平明显升高。BAMG-SF是一种很有前景的生物材料，可用于ASCs种植，促进膀胱扩张，并显示出增强的血管生成潜力，可能与通过ERK 1/2激活SDF-1α/CXCR4途径有关。
The unfavourable clinical outcomes of host cell-seeded scaffolds for bladder augmentation warrant improved bioactive biomaterials. This study aimed to examine the feasibility of adipose-derived stem cells (ASCs)-seeded bilayer bladder acellular matrix graft (BAMG)-silk fibroin (SF) scaffold in enhancing bladder reconstruction. Sprague Dawley rats were randomly divided into three groups: the BAMG-SF-ASCs group, the acellular BAMG-SF group and the cystotomy group. The BAMG-SF-ASCs group was sampled at 2, 4 and 12 weeks, and compared with the other groups at 12 weeks. In the BAMG-SF-ASCs group, the normal bladder contour was reformed similar to that in the cystotomy group, with abundant urothelium and smooth muscle regeneration, as well as a suitable scaffold degradation speed, and trivial fibrosis and inflammation. The ASCs seeded in BAMG-SF were maintained in the regenerated region during the 12-week experimental period and significantly enhanced the vessel density, nerve regeneration and bladder function compared with acellular BAMG-SF. In addition, the BAMG-SF-ASCs group presented elevated levels of SDF-1α, VEGF and their receptors, with an obvious increase in ERK 1/2 phosphorylation. BAMG-SF is a promising biomaterial for ASCs seeding to facilitate bladder augmentation and demonstrated an enhanced angiogenic potential possibly related to the SDF-1α/CXCR4 pathway via ERK 1/2 activation.
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影响因子：
1.2
作者：
Adamowicz J;Kowalczyk T;Drewa T
通讯作者：
Drewa T
影响因子：
14
作者：
Sharma, Arun K.;Hota, Partha V.;Cheng, Earl Y.
通讯作者：
Cheng, Earl Y.
DOI：
10.4111/kju.2014.55.4.228
发表时间：
2014-04
期刊：
Korean journal of urology
影响因子：
--
作者：
Kim JH;Lee HJ;Song YS
通讯作者：
Song YS
影响因子：
37.8
作者：
Rehman, J;Traktuev, D;March, KL
通讯作者：
March, KL
影响因子：
6.6
作者：
Joseph, David B.;Borer, Joseph G.;McLorie, Gordon A.
通讯作者：
McLorie, Gordon A.