Fungal microbiota dysbiosis in IBD.
Fungal microbiota dysbiosis in IBD.
复制标题
DOI：
10.1136/gutjnl-2015-310746
复制
发表时间：
2017-06
期刊：
影响因子：
24.5
通讯作者：
Beaugerie L
中科院分区：
文献类型：
--
作者：
Sokol H;Leducq V;Aschard H;Pham HP;Jegou S;Landman C;Cohen D;Liguori G;Bourrier A;Nion-Larmurier I;Cosnes J;Seksik P;Langella P;Skurnik D;Richard ML;Beaugerie L
肠道菌群在人体生理学和IBD中起着重要作用。虽然一些数据表明真菌微生物群在IBD发病机制中的作用，但可用的数据很少。我们研究的目的是检测IBD患者粪便真菌菌群。分别使用16 S和ITS 2测序确定235名IBD患者和38名健康受试者（HS）的粪便微生物群的细菌和真菌组成。使用Qime管道分析获得的序列，以评估组成和多样性。与临床参数相关的细菌和真菌分类群使用线性模型的多变量关联来鉴定。使用斯皮尔曼检验和距离相关性研究细菌和真菌微生物群之间的相关性。我们观察到，真菌微生物群在IBD中是倾斜的，与HS相比，具有增加的担子菌/子囊菌的比例，减少的酿酒酵母的比例和增加的白色念珠菌的比例。我们还确定了疾病特异性的多样性改变，表明克罗恩病特异性肠道环境可能有利于真菌，而牺牲细菌。细菌和真菌微生物群的伴随分析显示HS中的密集和同质相关网络，但IBD中的网络显著不平衡，表明存在疾病特异性的界间改变。除了细菌生态失调外，我们的研究还确定了IBD中一种独特的真菌微生物群生态失调，其特征在于生物多样性和组成的改变。此外，我们在这里揭示了IBD中疾病特异性的王国间网络改变，表明除了细菌之外，真菌也可能在IBD发病机制中发挥作用。
The bacterial intestinal microbiota plays major roles in human physiology and IBDs. Although some data suggest a role of the fungal microbiota in IBD pathogenesis, the available data are scarce. The aim of our study was to characterise the faecal fungal microbiota in patients with IBD. Bacterial and fungal composition of the faecal microbiota of 235 patients with IBD and 38 healthy subjects (HS) was determined using 16S and ITS2 sequencing, respectively. The obtained sequences were analysed using the Qiime pipeline to assess composition and diversity. Bacterial and fungal taxa associated with clinical parameters were identified using multivariate association with linear models. Correlation between bacterial and fungal microbiota was investigated using Spearman's test and distance correlation. We observed that fungal microbiota is skewed in IBD, with an increased Basidiomycota/Ascomycota ratio, a decreased proportion of Saccharomyces cerevisiae and an increased proportion of Candida albicans compared with HS. We also identified disease-specific alterations in diversity, indicating that a Crohn's disease-specific gut environment may favour fungi at the expense of bacteria. The concomitant analysis of bacterial and fungal microbiota showed a dense and homogenous correlation network in HS but a dramatically unbalanced network in IBD, suggesting the existence of disease-specific inter-kingdom alterations. Besides bacterial dysbiosis, our study identifies a distinct fungal microbiota dysbiosis in IBD characterised by alterations in biodiversity and composition. Moreover, we unravel here disease-specific inter-kingdom network alterations in IBD, suggesting that, beyond bacteria, fungi might also play a role in IBD pathogenesis.
登录
查看更多内容
影响因子：
3.7
作者：
Cáliz R;Canet LM;Lupiañez CB;Canhão H;Escudero A;Filipescu I;Segura-Catena J;Soto-Pino MJ;Expósito-Ruiz M;Ferrer MÁ;García A;Romani L;González-Utrilla A;Vallejo T;Pérez-Pampin E;Hemminki K;Försti A;Collantes E;Fonseca JE;Sainz J
通讯作者：
Sainz J
酵母和细胞壁提取物对肠道炎症的调节：葡萄糖级分的应变依赖性和意外的抗炎作用。
影响因子：
3.7
作者：
Jawhara S;Habib K;Maggiotto F;Pignede G;Vandekerckove P;Maes E;Dubuquoy L;Fontaine T;Guerardel Y;Poulain D
通讯作者：
Poulain D
DOI：
10.1126/science.1221789
发表时间：
2012-06-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Iliev ID;Funari VA;Taylor KD;Nguyen Q;Reyes CN;Strom SP;Brown J;Becker CA;Fleshner PR;Dubinsky M;Rotter JI;Wang HL;McGovern DP;Brown GD;Underhill DM
通讯作者：
Underhill DM
影响因子：
30.3
作者：
Gevers D;Kugathasan S;Denson LA;Vázquez-Baeza Y;Van Treuren W;Ren B;Schwager E;Knights D;Song SJ;Yassour M;Morgan XC;Kostic AD;Luo C;González A;McDonald D;Haberman Y;Walters T;Baker S;Rosh J;Stephens M;Heyman M;Markowitz J;Baldassano R;Griffiths A;Sylvester F;Mack D;Kim S;Crandall W;Hyams J;Huttenhower C;Knight R;Xavier RJ
通讯作者：
Xavier RJ
影响因子：
3.7
作者：
Hoffmann C;Dollive S;Grunberg S;Chen J;Li H;Wu GD;Lewis JD;Bushman FD
通讯作者：
Bushman FD