Modulation of angiotensin II-induced vasoconstriction by endothelium-derived relaxing factor in the isolated microperfused rabbit afferent arteriole.
Modulation of angiotensin II-induced vasoconstriction by endothelium-derived relaxing factor in the isolated microperfused rabbit afferent arteriole.
复制标题
离体微灌注兔传入小动脉中内皮衍生舒张因子对血管紧张素 II 诱导的血管收缩的调节。
DOI：
10.1172/jci115181
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Carretero,OA
中科院分区：
文献类型：
--
作者：
Ito,S;Johnson,CS;Carretero,OA
虽然内皮源性舒张因子（EDRF）已被广泛研究，在大血管，很少有人知道它的作用，在肾小球前传入小动脉（Af-Art）。我们测试了这一假设，即EDRF，这是本地产生的Af-Art，调节小动脉血管紧张素II（AII）的反应。在体外以60 mmHg对肾小球完整的单个兔Af-Art进行微灌注。当首次应用0.1 μ M AII时，管腔直径减少49 +/- 7.0%，（n = 9; P小于0.0001）;然而，收缩减弱，在1分钟时减少15 +/- 3.5%。清洗Af-Art后，重复AII引起较少的收缩（13 +/- 4.0%; P小于0.0002，与首次应用相比），显示快速耐受。N-硝基-L-精氨酸（N-Arg）可抑制一氧化氮的合成（EDRF），预处理可使基底直径减少18 +/- 3.0%（n = 14; P <0.0001）。N-Arg还增强了AII诱导的收缩（86 +/- 6.8%;与未处理的Af-Art相比，P <0.02），并使其持续（1分钟时为82 +/- 6.9%）。即使在用N-Arg预处理后，重复AII引起较弱的反应，通过用富含血管紧张素酶的肾匀浆洗涤来恢复。总之，本研究提供的证据表明，局部产生的EDRF是一个重要的决定因素的基调Af-Art。我们的研究结果表明，AII诱导的Af-Art收缩的短暂性质可能是由于产生的EDRF，而快速耐受可能是长期持久的受体占用的结果。
Although endothelium-derived relaxing factor (EDRF) has been studied extensively in large vessels, little is known about its role in the preglomerular afferent arteriole (Af-Art). We tested the hypothesis that EDRF, which is produced locally in the Af-Art, modulates arteriolar responses to angiotensin II (AII). A single rabbit Af-Art with its glomerulus intact was microperfused in vitro at 60 mmHg. When 0.1 microM AII was first applied, luminal diameter decreased by 49 +/- 7.0% (n = 9; P less than 0.0001); however, constriction waned, with the decrease becoming 15 +/- 3.5% at 1 min. After washing the Af-Art, repeated AII caused less constriction (13 +/- 4.0%; P less than 0.0002 vs. first application), showing tachyphylaxis. Pretreatment with Nw-nitro-L-arginine (N-Arg), which inhibits synthesis of nitric oxide (an EDRF), decreased basal diameter by 18 +/- 3.0% (n = 14; P less than 0.0001). N-Arg also augmented AII-induced constriction (86 +/- 6.8%; P less than 0.02 vs. nontreated Af-Art) and rendered it persistent (82 +/- 6.9% at 1 min). Even after pretreatment with N-Arg, repeated AII caused a weaker response, which was restored by washing with kidney homogenate rich in angiotensinase. In conclusion, this study provides evidence that local production of EDRF is an important determinant of the tone of the Af-Art. Our results suggest that the transient nature of AII-induced constriction of the Af-Art may be due to production of EDRF, while tachyphylaxis may be the result of long lasting receptor occupancy.Images
登录
查看更多内容
血管紧张素 II 在分离和灌注的犬肠系膜动脉中诱导快速收缩。
DOI：
10.1620/tjem.150.417
发表时间：
1986
期刊：
The Tohoku journal of experimental medicine
影响因子：
--
作者：
S. Chiba;M. Tsukada
通讯作者：
M. Tsukada
血管紧张素快速耐受及其逆转
影响因子：
20.1
作者：
P. Khairallah;Irvine H. Page;F. Bumpus;R. Turker
通讯作者：
R. Turker
内皮源性舒张因子在调节肾血流动力学反应中的作用。
影响因子：
--
作者：
J. P. Tolins;R. Palmer;S. Moncada;L. Raij
通讯作者：
L. Raij
血管紧张素 II 和去甲肾上腺素对离体大鼠传入和传出小动脉的影响。
DOI：
10.1152/ajprenal.1990.258.3.f741
发表时间：
1990
期刊：
The American journal of physiology
影响因子：
--
作者：
Yuan,BH;Robinette,JB;Conger,JD
通讯作者：
Conger,JD
兔主动脉正常环和外翻环的血管紧张素快速耐受。
影响因子：
5
作者：
Eneida G. Silva;Alice T. Ferreira;Antonio CM. Paiva;Therezinha B. Paiva
通讯作者：
Therezinha B. Paiva