A biomarker-responsive T(2ex) MRI contrast agent.
A biomarker-responsive T(2ex) MRI contrast agent.
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DOI：
10.1002/mrm.26250
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发表时间：
2017-04
影响因子：
3.3
通讯作者：
Pagel, Mark D.
中科院分区：
文献类型：
--
作者：
Daryaei, Iman;Randtke, Edward A.;Pagel, Mark D.
本研究研究了一种全新的分子成像MRI造影剂，在与分子生物标志物相互作用后改变T2交换（T2ex）特性。对比剂Tm-DO3A-oAA用NO和O2处理。测定了反应物和生成物在浓度、温度和ph下的R1和R2弛豫率。使用7 T MRI扫描仪获取了反应物和生成物的CEST光谱，并分析了化学交换率和r2ex弛豫率。Tm-DO3A-oAA与NO、O2反应后，药剂的r2松驰率提高了6.4倍，而r1松驰率保持不变，表明Tm-DO3A-oAA是一种反应性强的T2ex药剂。pH和温度对反应物和生成物r2弛豫度的影响支持了产物的苯并咪唑配体相对于反应物的邻氨基苯胺配体交换速率较慢的化学交换速率较快的结论。T2ex MRI造影剂是一种新型的反应性造影剂，对检测生物标志物具有良好的检测敏感性和特异性，可作为分子成像的新工具。
This study investigated a fundamentally new type of responsive MRI contrast agent for molecular imaging that alters T2 exchange (T2ex) properties after interacting with a molecular biomarker. The contrast agent Tm-DO3A-oAA was treated with nitric oxide (NO) and O2. The R1 and R2 relaxation rates of the reactant and product were measured with respect to concentration, temperature and pH. CEST spectra of the reactant and product were acquired using a 7 T MRI scanner and analyzed to estimate chemical exchange rates and r2ex relaxivities. The reaction of Tm-DO3A-oAA with NO and O2 caused a 6.4-fold increase in the r2 relaxivity of the agent, while r1 relaxivity remained unchanged, which demonstrated that Tm-DO3A-oAA is a responsive T2ex agent. The effects of pH and temperature on the r2 relaxivities of the reactant and product supported the conclusion that the product’s benzimidazole ligand caused the agent to have a fast chemical exchange rate relative to the slow exchange rate of the reactant’s ortho-aminoanilide ligand. T2ex MRI contrast agents are a new type of responsive agent that have good detection sensitivity and specificity for detecting a biomarker, which can serve as a new tool for molecular imaging.
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影响因子：
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