Anticonvulsive effect of paeoniflorin on experimental febrile seizures in immature rats: possible application for febrile seizures in children.
Anticonvulsive effect of paeoniflorin on experimental febrile seizures in immature rats: possible application for febrile seizures in children.
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DOI：
10.1371/journal.pone.0042920
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Fukuda M
中科院分区：
文献类型：
--
作者：
Hino H;Takahashi H;Suzuki Y;Tanaka J;Ishii E;Fukuda M
热性惊厥（FS）是儿童中最常见的惊厥疾病，但目前还没有草药治疗FS的临床和实验研究，草药在亚洲国家广泛使用。芍药苷（Paeoniflorin， PF）是白芍的主要生物活性成分，含有PF的草药已被用于神经肌肉、神经精神和神经退行性疾病。在本研究中，我们分析了PF和Keishikashakuyaku-to （KS；一种含PF的草药）作为人类FS模型对未成熟大鼠高热诱发癫痫发作的抗惊厥作用。未成熟（P5）雄性大鼠给予PF或KS 10天后，与对照大鼠相比，高热诱发的癫痫发作明显受到抑制。在培养的海马神经元中，PF抑制谷氨酸诱导的细胞内Ca2+ ([Ca2+]i)升高、谷氨酸受体介导的膜去极化和谷氨酸诱导的神经元死亡。此外，PF部分抑制了代谢性谷氨酸受体5 （mGluR5）激活引起的[Ca2+]i升高，但不抑制α-氨基-3-羟基-5-甲基-4-异唑丙酸（AMPA）或n -甲基- d -天冬氨酸（NMDA）受体介导的[Ca2+]i升高。然而，PF不影响海马神经元γ-氨基丁酸（GABA）的产生和释放。这些结果表明，PF或含有PF的草药至少在一定程度上通过防止mglur5依赖性[Ca2+]i升高而发挥抗惊厥作用。因此，它可能是治疗儿童FS的可能候选药物。
Febrile seizures (FS) is the most common convulsive disorder in children, but there have been no clinical and experimental studies of the possible treatment of FS with herbal medicines, which are widely used in Asian countries. Paeoniflorin (PF) is a major bioactive component of Radix Paeoniae alba, and PF-containing herbal medicines have been used for neuromuscular, neuropsychiatric, and neurodegenerative disorders. In this study, we analyzed the anticonvulsive effect of PF and Keishikashakuyaku-to (KS; a PF-containing herbal medicine) for hyperthermia-induced seizures in immature rats as a model of human FS. When immature (P5) male rats were administered PF or KS for 10 days, hyperthermia-induced seizures were significantly suppressed compared to control rats. In cultured hippocampal neurons, PF suppressed glutamate-induced elevation of intracellular Ca2+ ([Ca2+]i), glutamate receptor-mediated membrane depolarization, and glutamate-induced neuronal death. In addition, PF partially suppressed the elevation in [Ca2+]i induced by activation of the metabotropic glutamate receptor 5 (mGluR5), but not that mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolpropionic acid (AMPA) or N-methyl-D-aspartate (NMDA) receptors. However, PF did not affect production or release of γ-aminobutyric acid (GABA) in hippocampal neurons. These results suggest that PF or PF-containing herbal medicines exert anticonvulsive effects at least in part by preventing mGluR5-dependent [Ca2+]i elevations. Thus, it could be a possible candidate for the treatment of FS in children.
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影响因子：
3.7
作者：
Hiratsuka, Toru;Matsuzaki, Shinsuke;Tohyama, Masaya
通讯作者：
Tohyama, Masaya
影响因子：
--
作者：
Nakanishi, S;Nakajima, Y;Okada, M
通讯作者：
Okada, M
影响因子：
4
作者：
Mao, Qing-Qiu;Zhong, Xiao-Ming;Huang, Zhen
通讯作者：
Huang, Zhen
DOI：
10.1016/0165-3806(91)90118-3
发表时间：
1991-07-16
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
BARAM, TZ;SCHULTZ, L
通讯作者：
SCHULTZ, L
影响因子：
2.2
作者：
HJERESEN, DL;DIAZ, J
通讯作者：
DIAZ, J