Association of Bordetella dermonecrotic toxin with the extracellular matrix.
Association of Bordetella dermonecrotic toxin with the extracellular matrix.
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DOI：
10.1186/1471-2180-10-247
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发表时间：
2010-09-25
期刊：
影响因子：
4.2
通讯作者：
Horiguchi Y
中科院分区：
文献类型：
--
作者：
Fukui-Miyazaki A;Kamitani S;Miyake M;Horiguchi Y
博德泰拉皮损毒素（DNT）通过抑制成骨细胞分化导致猪支气管脓毒杆菌感染引起的猪萎缩性鼻炎的鼻甲萎缩。这种毒素不会主动从细菌中分泌出来，据推测，在感染区域只有少量的毒素存在。如此小的剂量如何影响目标组织尚不清楚。荧光显微镜显示，DNT与培养细胞上形成的纤维结构相关。一种与DNT交联的细胞成分通过质谱鉴定为纤维连接蛋白。荧光显微镜还观察了DNT细胞上纤维连接蛋白网络的共定位。一些证据表明DNT与纤维连接蛋白不是直接相互作用，而是通过另一种尚未确定的细胞成分相互作用。共定位不仅在dnt敏感细胞中观察到，而且在不敏感细胞中也观察到，这表明纤维连接蛋白网络既不作为毒素的受体，也不参与中毒过程。当局部环境中毒素浓度降低时，纤维连接蛋白网络相关毒素容易被释放，并保持活性。众所周知，细胞外基质中的成分通过结合和释放各种生长因子来调节细胞外环境的变化。同样，基于纤维连接蛋白的细胞外基质可以作为DNT的临时存储系统，使少量毒素能够有效地影响目标组织或细胞。
Bordetella dermonecrotic toxin (DNT) causes the turbinate atrophy in swine atrophic rhinitis, caused by a Bordetella bronchiseptica infection of pigs, by inhibiting osteoblastic differentiation. The toxin is not actively secreted from the bacteria, and is presumed to be present in only small amounts in infected areas. How such small amounts can affect target tissues is unknown. Fluorescence microscopy revealed that DNT associated with a fibrillar structure developed on cultured cells. A cellular component cross-linked with DNT conjugated with a cross-linker was identified as fibronectin by mass spectrometry. Colocalization of the fibronectin network on the cells with DNT was also observed by fluorescence microscope. Several lines of evidence suggested that DNT interacts with fibronectin not directly, but through another cellular component that remains to be identified. The colocalization was observed in not only DNT-sensitive cells but also insensitive cells, indicating that the fibronectin network neither serves as a receptor for the toxin nor is involved in the intoxicating procedures. The fibronectin network-associated toxin was easily liberated when the concentration of toxin in the local environment decreased, and was still active. Components in the extracellular matrix are known to regulate activities of various growth factors by binding and liberating them in response to alterations in the extracellular environment. Similarly, the fibronectin-based extracellular matrix may function as a temporary storage system for DNT, enabling small amounts of the toxin to efficiently affect target tissues or cells.
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影响因子：
11.4
作者：
Masuda, M;Betancourt, L;Horiguchi, Y
通讯作者：
Horiguchi, Y
影响因子：
2.5
作者：
Ho, Matthew S. P.;Boese, Kerstin;Smyth, Neil
通讯作者：
Smyth, Neil
影响因子：
3.1
作者：
Brockmeier, SL;Register, KB;Kunkle, RA
通讯作者：
Kunkle, RA
DOI：
10.1073/pnas.94.21.11623
发表时间：
1997-10-14
影响因子：
11.1
作者：
Horiguchi, Y;Inoue, N;Matsuda, M
通讯作者：
Matsuda, M
DOI：
10.1111/j.1574-695x.1995.tb00170.x
发表时间：
1995-09-01
影响因子：
--
作者：
HORIGUCHI, Y;OKADA, T;MATSUDA, M
通讯作者：
MATSUDA, M