A novel biosensor with high signal-to-noise ratio for real-time measurement of dopamine levels in vivo.
A novel biosensor with high signal-to-noise ratio for real-time measurement of dopamine levels in vivo.
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DOI：
10.1002/jnr.24193
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发表时间：
2018-05
影响因子：
4.2
通讯作者：
Hida H
中科院分区：
文献类型：
--
作者：
Ishida A;Imamura A;Ueda Y;Shimizu T;Marumoto R;Jung CG;Hida H
快速扫描循环伏安法（FSCV）是一种实时测量大脑中多巴胺（DA）水平的方法。然而，很难将DA与其他单胺如血清素（5‐羟色胺，5‐HT）和去甲肾上腺素（NE）区分开来。我们报道了一种新型的DA特异性生物传感器，由涂有离子交换膜的碳纤维电极、含有单胺氧化酶B的层和纤维素膜组成。我们使用探针进行FSCV检测体外和体内DA的量。首先，我们在磷酸盐缓冲盐水中分别加入1微摩尔的DA、5 - HT和NE，测量体外电流。结果证实了该生物传感器对DA的选择性检测。接下来，我们将探针植入雄性大鼠纹状体，研究其是否能选择性检测体内DA含量的变化。探针检测到甲基苯丙胺引起的强直性变化和电刺激内侧前脑束引起的相位变化。相比之下，6 -羟多巴胺损伤纹状体中的电极对系统性选择性5 -羟色胺或5 -羟色胺/去甲肾上腺素再摄取抑制剂没有反应，证实了其选择性。纹状体探针在电极植入1周后仍能检测到DA水平的变化。结果表明，这种新型生物传感器能够以较高的信噪比实时测量体内DA水平的变化。
Fast‐scan cyclic voltammetry (FSCV) is an established method for measuring dopamine (DA) levels in the brain in real time. However, it is difficult to discriminate DA from other monoamines such as serotonin (5‐hydroxytryptamine, 5‐HT) and norepinephrine (NE). We report a novel DA‐specific biosensor consisting of a carbon‐fiber electrode coated with an ion‐exchange membrane, a layer containing monoamine oxidase B, and a cellulose membrane. We performed FSCV using the probe to monitor the amount of DA in vitro and in vivo. First, we measured currents in vitro in phosphate‐buffered saline as we added one micromole each of DA, 5‐HT, and NE. The results confirmed that the biosensor selectively detected DA. Next, we implanted the probe in the striatum of male rats to investigate whether it could selectively detect changes in the DA content in vivo. The probe detected both the tonic change induced by methamphetamine administration and the phasic change induced by electrical stimulation of the medial forebrain bundle. In contrast, the electrode in the 6‐hydroxydopamine–lesioned striatum did not respond to systemic selective serotonin or serotonin/norepinephrine reuptake inhibitors, confirming its selectivity. Furthermore, the probe in the striatum could still detect changes in the DA level 1 week after electrode implantation. The results suggest that the novel biosensor can measure real‐time changes in DA levels in vivo with a relatively high signal‐to‐noise ratio.
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影响因子：
4.2
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
通讯作者：
Wightman, RM
影响因子：
3
作者：
KOVACH, PM;EWING, AG;WIGHTMAN, RM
通讯作者：
WIGHTMAN, RM
影响因子：
7.4
作者：
Jung, MC;Shi, GY;Weber, SG
通讯作者：
Weber, SG
影响因子：
3
作者：
CRESPI, F;ENGLAND, TG;TRIST, DG
通讯作者：
TRIST, DG
DOI：
10.1124/jpet.300.2.621
发表时间：
2002-02-01
影响因子：
3.5
作者：
Morisset, S;Pilon, C;Arrang, JM
通讯作者：
Arrang, JM