Localization of caveolin-1 and c-src in mature and differentiating photoreceptors: raft proteins co-distribute with rhodopsin during development.
Localization of caveolin-1 and c-src in mature and differentiating photoreceptors: raft proteins co-distribute with rhodopsin during development.
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DOI：
10.1007/s10735-011-9360-4
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发表时间：
2011-12
影响因子：
3.2
通讯作者：
Kiss, Anna L.
中科院分区：
文献类型：
--
作者：
Berta, Agnes I.;Boesze-Battaglia, Kathleen;Attila Magyar;Agoston Szel;Kiss, Anna L.
大量的生物化学和形态学研究提供了深入了解小窝蛋白-1的分布模式和膜筏在脊椎动物视网膜中的存在。然而，到目前为止，研究还没有解决的筏特异性蛋白质在发育过程中的定位概况。因此，我们的研究目的是跟踪小窝蛋白-1，磷酸小窝蛋白-1和c-src在发育中的视网膜中的定位模式，并将其与在成人中观察到的模式进行比较。特异性抗体用于可视化小窝蛋白-1、c-src、磷酸化小窝蛋白-1和磷酸化小窝蛋白-1的激酶的分布。将该支架复合物的标记模式与视紫红质和视紫红质激酶的标记模式进行比较。在出生后发育期间的不同时间点分析样品，并与成人视网膜进行比较。免疫细胞化学研究补充免疫印迹和免疫沉淀研究。在成熟的视网膜caveolin-1和c-src主要定位于细胞体和IS的光感受器，只有非常弱的标记OS。相比之下，磷酸-caveolin-1是只检测到在OS的光感受器。在开发过程中，我们遵循这些蛋白质的表达和分布曲线的时间序列，特别注意OS形成最强大的时期。免疫细胞化学和免疫沉淀双标记显示视紫红质与caveolin-1和c-src共定位和共沉淀。在P10时，外界膜和外丛状层之间的单个点状结构被视紫红质和小窝蛋白-1以及视紫红质和c-src分别标记。这些研究表明，膜筏特异性蛋白质在发育过程中是共分布的，从而指出了这种复合物在OS形成中的作用。此外，含有小窝蛋白-1，c-src和视紫红质的小点状结构的存在提高了这些蛋白质在发育过程中可能一起转运到OS的可能性，并且小窝蛋白-1主要以磷酸化形式存在于OS中。
Numerous biochemical and morphological studies have provided insight into the distribution pattern of caveolin-1 and the presence of membrane rafts in the vertebrate retina. To date however, studies have not addressed the localization profile of raft specific proteins during development. Therefore the purpose of our studies was to follow the localization pattern of caveolin-1, phosphocaveolin-1 and c-src in the developing retina and compare it to that observed in adults. Specific antibodies were used to visualize the distribution of caveolin-1, c-src, a kinase phosphorylating caveolin-1, and phospho-caveolin-1. The labeling pattern of this scaffolded complex was compared to those of rhodopsin and rhodopsin kinase. Samples were analyzed at various time points during postnatal development and compared to adult retinas. The immunocytochemical studies were complemented with immunoblots and immunoprecipitation studies. In the mature retina caveolin-1 and c-src localized mainly to the cell body and IS of photoreceptors, with only very weakly labeled OS. In contrast, phospho-caveolin-1 was only detectable in the OS of photoreceptors. During development we followed the expression and distribution profile of these proteins in a temporal sequence with special attention to the period when OS formation is most robust. Double labeling immunocytochemistry and immunoprecipitation showed rhodopsin to colocalize and co-immunoprecipitate with caveolin-1 and c-src. Individual punctate structures between the outer limiting membrane and the outer plexiform layer were seen at P10 to be labeled by both rhodopsin and caveolin-1 as well as by rhodopsin and c-src, respectively. These studies suggest that membrane raft specific proteins are co-distributed during development, thereby pointing to a role for such complexes in OS formation. In addition, the presence of small punctate structures containing caveolin-1, c-src and rhodopsin raise the possibility that these proteins may transport together to OS during development and that caveolin-1 exists predominantly in a phosphorylated form in the OS.
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DOI：
10.1083/jcb.200806009
发表时间：
2008-11-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Baker SA;Haeri M;Yoo P;Gospe SM 3rd;Skiba NP;Knox BE;Arshavsky VY
通讯作者：
Arshavsky VY
影响因子：
4.8
作者：
Nair, KS;Hanson, SM;Slepak, VZ
通讯作者：
Slepak, VZ
影响因子：
2
作者：
ROHLICH, P;SZEL, A
通讯作者：
SZEL, A
影响因子：
4.7
作者：
Elliott, Michael H.;Nash, Zack A.;Naash, Muna I.
通讯作者：
Naash, Muna I.
影响因子：
1.9
作者：
Calderone, JB;Jacobs, GH
通讯作者：
Jacobs, GH