Regulation of pro-gonadotropin-releasing hormone gene expression by sex steroids in the brain of male and female rats.
Regulation of pro-gonadotropin-releasing hormone gene expression by sex steroids in the brain of male and female rats.
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雄性和雌性大鼠大脑中性类固醇对促性腺激素释放激素基因表达的调节。
DOI：
10.1210/mend-3-11-1748
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发表时间：
1989
影响因子：
--
通讯作者：
G. Pelletier
中科院分区：
文献类型：
--
作者：
D. Toranzo;É. Dupont;J. Simard;C. Labrie;J. Couet;F. Labrie;G. Pelletier
性激素对促性腺激素基因表达和分泌的精细调节是公认的，它是通过直接作用于垂体前叶水平和调节下丘脑水平GnRH分泌的脉动模式来调节的。由于性激素对下丘脑GnRH基因表达水平的影响尚不清楚，本研究采用定量原位杂交技术，研究了性激素对成年大鼠细胞内GnRH原基因表达水平的影响。观察了去性腺14天后，每日两次给予17β-雌二醇(E_2，0.25微克)或双氢睾酮(DHT，100微克)对去性腺动物的影响。此外，还研究了单独使用黄体酮(P，2毫克，每天两次)或在雌激素存在的情况下，对去卵巢动物的影响。用35S标记的编码大鼠促性腺激素释放激素原的cDNA探针进行杂交，通过计数覆盖在标记神经元上的银粒数量来评估相应的mRNA水平。在雄性大鼠中，去势导致每个神经元的平均颗粒数(与正常大鼠相比)显著增加65%。给去势动物注射E2或DHT可完全阻止去势后促性腺激素释放激素原基因表达水平的升高。在雌性动物中，卵巢切除的效果不如雄性动物显著，增加了25%(P<0.001)。雌二醇组和双羟色胺组可完全阻止去卵巢引起的促性腺激素释放激素原基因表达水平的升高。此外，在去卵巢的动物中，P显著增强了E_2对促性腺激素释放激素原mRNA水平的抑制作用。
The fine modulation of gonadotropin gene expression and secretion is well recognized to be regulated by sex steroids through their direct action both at the anterior pituitary level and on the pulsatile pattern of GnRH secretion at the hypothalamic level. Since the influence of sex steroids on hypothalamic GnRH mRNA levels remains to be elucidated, quantitative in situ hybridization was used to study the effect of sex steroids on cellular levels of pro-GnRH mRNA in adult rats of both sexes. The effects of 14-day gonadectomy as well as administration of 17 beta-estradiol (E2, 0.25 micrograms) or dihydrotestosterone (DHT, 100 micrograms) twice a day during 14 days to gonadectomized animals were evaluated. In addition, the effect of progesterone (P, 2 mg, twice daily) alone or in the presence of E2 was also studied in ovariectomized animals. Hybridization was performed using a 35S-labeled cDNA probe encoding rat pro-GnRH and the corresponding mRNA levels were assessed by counting the number of silver grains overlying labeled neurons. In male rats, castration induced a highly significant 65% increase (compared to intact rats) in the mean number of grains per neuron. Administration of E2 or DHT to castrated animals completely prevented the post castration rise in pro-GnRH mRNA levels. In female animals, the effect of ovariectomy was less striking than in the male, a 25% increase (P less than 0.001) being observed. Treatment with E2 or DHT also completely prevented the increase in pro-GnRH mRNA levels induced by ovariectomy. Moreover, treatment with P in ovariectomized animals markedly potentiated the inhibitory effect of E2 on pro-GnRH mRNA levels.(ABSTRACT TRUNCATED AT 250 WORDS)
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雄性大鼠大脑中促性腺激素释放激素和阿片黑皮质素原基因表达的青春期变化。
影响因子：
4.8
作者：
Wiemann,JN;Clifton,DK;Steiner,RA
通讯作者：
Steiner,RA
DOI：
10.1056/nejm198612043152306
发表时间：
1986-12
期刊：
The New England journal of medicine
影响因子：
--
作者：
J. Marshall;R. Kelch
通讯作者：
J. Marshall;R. Kelch
雄性大鼠黄体生成素释放激素神经元的形态与年龄和激素状况的函数关系。
影响因子：
4.1
作者：
Witkin,JW
通讯作者：
Witkin,JW
通过原位杂交组织化学对灵长类动物大脑中含有促性腺激素释放激素信使核糖核酸的细胞进行神经解剖学定位。
DOI：
10.1210/mend-1-5-371
发表时间：
1987
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Standish,LJ;Adams,LA;Vician,L;Clifton,DK;Steiner,RA
通讯作者：
Steiner,RA
影响因子：
56.9
作者：
LUBAHN, DB;JOSEPH, DR;WILSON, EM
通讯作者：
WILSON, EM