Effect of an omega-3 lipid emulsion in reducing oxidative stress in a rat model of intestinal ischemia-reperfusion injury.
Effect of an omega-3 lipid emulsion in reducing oxidative stress in a rat model of intestinal ischemia-reperfusion injury.
复制标题
DOI：
10.1007/s00383-012-3144-0
复制
发表时间：
2012-09
影响因子：
1.8
通讯作者：
Morikawa Y
中科院分区：
文献类型：
--
作者：
Arisue A;Shimojima N;Tomiya M;Shimizu T;Harada D;Nakayama M;Tomita H;Shinoda M;Tanabe M;Maruyama I;Mizuno M;Kuroda T;Wakabayashi G;Morikawa Y
已对omega-3脂肪乳剂的有效性进行了广泛研究。本研究的目的是检查omega-3脂肪乳剂在肠缺血再灌注损伤大鼠模型中降低氧化应激的作用及其潜在机制。将66只大鼠分为三个饮食组（无脂、大豆油和鱼油组）。每只动物给予全胃肠外营养3天，然后诱导肠缺血100分钟。接受假手术的动物作为对照。分别于术后6 h和12 h采集肠组织和血液，进行组织学损伤评分、血浆相关参数评价和统计学评价。鱼油组肠组织的组织学损伤评分显著低于大豆油组（P = 0.0121）。鱼油组的晚相尿8-羟基-2-脱氧鸟苷水平也显著低于其他组（P = 0.0267）。此外，高迁移率族蛋白1蛋白的血浆水平在鱼油组中也显著低于无脂质组（P = 0.0398）。在缺血再灌注损伤前静脉注射omega-3脂肪乳剂似乎可降低氧化应激和组织损伤的严重程度。膜脂肪酸的修饰可能是减少组织损伤的机制。
The usefulness of omega-3 lipid emulsions has been extensively studied. The objectives of the present study were to examine the effect of an omega-3 lipid emulsion in reducing oxidative stress in a rat model of intestinal ischemia−reperfusion injury and the underlying mechanism. A total of 66 rats were divided into three dietary groups (lipid-free, soybean oil, and fish oil groups). Each animal was administered total parenteral nutrition for 3 days, followed by induction of intestinal ischemia for 100 min. Animals subjected to sham surgery served as the controls. Intestinal tissue and blood were harvested 6 and 12 h after the surgery, then, assessment of the histological damage score, plasma-related parameters, and statistical evaluation were performed. The histological damage score in the intestinal tissues was significantly lower in the fish oil group than in the soybean oil group (P = 0.0121). The late-phase urinary level of 8-hydroxy-2-deoxyguanosine was also significantly lower in the fish oil group as compared with that in the other groups (P = 0.0267). Furthermore, the plasma level of high-mobility group box 1 protein was also significantly lower in the fish oil group as compared with that in the lipid-free group (P = 0.0398). It appeared that intravenous administration of an omega-3 lipid emulsion prior to ischemia−reperfusion injury reduced the oxidative stress and severity of tissue damage. Modification of membrane fatty acids may serve as the mechanism underlying this reduction of tissue damage.
登录
查看更多内容
DOI：
10.1164/ajrccm.164.10.2106117
发表时间：
2001-11-15
影响因子：
24.7
作者：
Wang, HH;Yang, H;Tracey, KJ
通讯作者：
Tracey, KJ
影响因子：
2.2
作者：
Shimojima, Naoki;Nakaki, Toshio;Kitajima, Masaki
通讯作者：
Kitajima, Masaki
DOI：
10.1097/mpg.0b013e318182c8f6
发表时间：
2009-02-01
影响因子：
2.9
作者：
Diamond, Ivan R.;Sterescu, Anca;Wales, Paul W.
通讯作者：
Wales, Paul W.
DOI：
10.1016/j.jtcvs.2010.04.030
发表时间：
2011-03-01
影响因子：
6
作者：
Byrne, John;McGuinness, Jonathan;Redmond, Mark J.
通讯作者：
Redmond, Mark J.
影响因子：
4.5
作者：
Liu, Hongyan;Mctaggart, Steven J.;Gobe, Glenda C.
通讯作者：
Gobe, Glenda C.