GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate.
GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate.
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DOI：
10.1126/sciadv.abg0834
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发表时间：
2022-03-11
期刊：
影响因子：
13.6
通讯作者：
Scott IC
中科院分区：
文献类型：
--
作者：
Song M;Yuan X;Racioppi C;Leslie M;Stutt N;Aleksandrova A;Christiaen L;Wilson MD;Scott IC
GATA4/5/6转录因子在心脏发育中起着重要的、保守的作用。为了了解GATA4/5/6如何调控中胚层到心脏的命运转变，我们标记、分离并对从斑马鱼原肠形成到体细胞发生的心脏前时间点表达GATA5的细胞进行了单细胞基因表达分析。我们发现，大多数中胚层来源的谱系都有GATA5/6的动态表达。在没有Gata5/6的情况下，中内胚层来源的细胞的群体结构发生了实质性的变化。除了预期的心脏中胚层缺失外，我们证实了伴随而来的颅咽中胚层扩张。此外，Gata5/6缺失导致心脏和咽部基因附近染色质可及性的广泛变化。斑马鱼和被囊状乔纳只有一个GATA4/5/6同源物的功能分析表明，GATA4/5/6作用于TBX1的上游，在促进心脏和抑制咽中胚层的识别方面发挥必要的细胞自主作用。总体而言，心脏和咽中胚层的命运选择是通过进化上保守的GATA4/5/6调控网络实现的。心脏和咽部命运的决定由古老的GATA4/5/6调节网络平衡。
GATA4/5/6 transcription factors play essential, conserved roles in heart development. To understand how GATA4/5/6 modulates the mesoderm-to-cardiac fate transition, we labeled, isolated, and performed single-cell gene expression analysis on cells that express gata5 at precardiac time points spanning zebrafish gastrulation to somitogenesis. We found that most mesendoderm-derived lineages had dynamic gata5/6 expression. In the absence of Gata5/6, the population structure of mesendoderm-derived cells was substantially altered. In addition to the expected absence of cardiac mesoderm, we confirmed a concomitant expansion of cranial-pharyngeal mesoderm. Moreover, Gata5/6 loss led to extensive changes in chromatin accessibility near cardiac and pharyngeal genes. Functional analyses in zebrafish and the tunicate Ciona, which has a single GATA4/5/6 homolog, revealed that GATA4/5/6 acts upstream of tbx1 to exert essential and cell-autonomous roles in promoting cardiac and inhibiting pharyngeal mesoderm identity. Overall, cardiac and pharyngeal mesoderm fate choices are achieved through an evolutionarily conserved GATA4/5/6 regulatory network. Cardiac and pharyngeal fate decisions are balanced by an ancient GATA4/5/6 regulatory network.
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影响因子：
14.9
作者：
The Gene Ontology Consortium
通讯作者：
The Gene Ontology Consortium
影响因子：
--
作者：
Christiaen, Lionel;Wagner, Eileen;Levine, Michael
通讯作者：
Levine, Michael
影响因子：
2.7
作者：
Beuling, Eva;Bosse, Tjalling;aan de Kerk, Daniel J.;Piaseckyj, Christina M.;Fujiwara, Yuko;Katz, Samuel G.;Orkin, Stuart H.;Grand, Richard J.;Krasinski, Stephen D.
通讯作者：
Krasinski, Stephen D.
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
DOI：
10.1088/1742-5468/2008/10/p10008
发表时间：
2008-10-01
影响因子：
2.4
作者：
Blondel, Vincent D.;Guillaume, Jean-Loup;Lefebvre, Etienne
通讯作者：
Lefebvre, Etienne