Diaphragm activation via high frequency spinal cord stimulation in a rodent model of spinal cord injury.
Diaphragm activation via high frequency spinal cord stimulation in a rodent model of spinal cord injury.
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DOI：
10.1016/j.expneurol.2013.03.006
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发表时间：
2013-09
影响因子：
5.3
通讯作者：
DiMarco, Anthony F.
中科院分区：
文献类型：
--
作者：
Kowalski, Krzysztof E.;Hsieh, Yee-Hsee;Dick, Thomas E.;DiMarco, Anthony F.
如在犬模型中所证明的，与当前技术相比，高频脊髓刺激（HF-SCS）是一种新颖且更生理的吸气肌电激活方法。然而，由于成本和社会问题，狗模型具有显著的局限性。由于啮齿动物呼吸系统也是研究神经元回路功能的相关模型，因此本研究的目的是a）评估HF-SCS的作用和B）确定该技术在大鼠中的应用方法。在9只Sprague道利大鼠中，在C1脊髓切片后在T2水平的脊髓腹侧硬膜外表面上应用自主呼吸和HF-SCS期间评估了膈肌多单位和单运动单位EMG活动。在犬中，HF-SCS导致隔膜在生理放电频率下激活并产生大的吸入量。自主呼吸和HF-SCS期间膈肌的平均最大放电频率分别为23.3 ± 1.4 Hz（范围：9.8 - 51.6 Hz）和26.6 ± 1.3 Hz;范围：12.0 - 72.9 Hz，吸气量相当。此外，HF-SCS在60分钟内成功起搏这些动物，没有系统疲劳的证据。我们的研究结果表明，类似的狗模型，HF-SCS在大鼠的结果在激活脊髓束突触与膈运动神经元池，允许处理的刺激和随之而来的生理激活的吸气肌。该大鼠为进一步研究膈运动神经元生理学提供了一个有用的模型。
As demonstrated in a canine model, high frequency spinal cord stimulation (HF-SCS) is a novel and more physiologic method of electrical activation of the inspiratory muscles compared to current techniques. The dog model, however, has significant limitations due to cost and societal concerns. Since the rodent respiratory system is also a relevant model for study of neuronal circuitry function, the aims of the present study were to a) assess the effects of HF-SCS and b) determine the methodology of application of this technique in rats. In 9 Sprague Dawley rats, diaphragm multiunit and single motor unit EMG activity was assessed during spontaneous breathing and HF-SCS applied on the ventral epidural surface of the spinal cord at the T2 level following C1 spinal section. As in dogs, HF-SCS results in the activation of the diaphragm at physiological firing frequencies and the generation of large inspired volumes. Mean maximum firing frequencies of the diaphragm during spontaneous breathing and HF-SCS were 23.3 ± 1.4 Hz (range: 9.8 – 51.6 Hz) and 26.6 ± 1.3 Hz; range: 12.0 – 72.9 Hz, respectively, at comparable inspired volumes. Moreover, HF-SCS was successful in pacing these animals over a 60-min period without evidence of system fatigue. Our results suggest that, similar to the dog model, HF-SCS in the rat results in activation of spinal cord tracts which synapse with the phrenic motoneuron pool, allowing processing of the stimulus and consequent physiologic activation of the inspiratory muscles. The rat may be a useful model for further studies evaluating phrenic motoneuron physiology.
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影响因子：
5.5
作者：
de Almeida, Anoushka T. R.;Al-Izki, Sarah;Kirkwood, Peter A.
通讯作者：
Kirkwood, Peter A.
影响因子：
2.5
作者：
HENNEMAN, E;SOMJEN, G;CARPENTER, DO
通讯作者：
CARPENTER, DO
影响因子：
9.6
作者：
Mink, SN;Gonzalez, X;Tan, L
通讯作者：
Tan, L
影响因子：
15.9
作者：
Lane, Michael A.;Fuller, David D.;White, Todd E.;Reier, Paul J.
通讯作者：
Reier, Paul J.
DOI：
10.1164/ajrccm/136.6.1385
发表时间：
1987-12-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
DIMARCO, AF;ALTOSE, MD;DURAND, D
通讯作者：
DURAND, D