Characterization of a novel plasmid type and various genetic contexts of bla OXA-58 in Acinetobacter spp. from multiple cities in China.
Characterization of a novel plasmid type and various genetic contexts of bla OXA-58 in Acinetobacter spp. from multiple cities in China.
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不动杆菌属中 bla(OXA-58) 的新型质粒类型和各种遗传背景的表征。
DOI：
10.1371/journal.pone.0084680
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Fu Y;Jiang J;Zhou H;Jiang Y;Fu Y;Yu Y;Zhou J
已有多项研究描述了中国地区携带OXA-58不动杆菌的流行病学分布情况。然而，关于不动杆菌携带bla OXA-58的质粒的复制子类型和bla OXA-58周围的遗传背景的数据有限。在中国。12株非复制BLAOXA-58-不动杆菌属。2005年至2010年期间，从五个不同城市的六家医院收集了分离株。采用脉冲场凝胶电泳法和多位点序列分型方法对分离株进行分子流行病学研究。用聚合酶链式反应鉴定碳青霉烯酶编码基因和质粒复制酶基因。用S1-核酸酶方法对bla OXA-58进行了遗传定位分析。通过质粒接合和电转化鉴定携带BLA OXA-58的重组表达载体的转移性。通过克隆实验确定了Bla OXA-58的遗传结构。这12株细菌包括2株垂体不动杆菌(属于1个脉冲型)、3株院内不动杆菌(属于2个脉冲型)和7株鲍曼不动杆菌(属于2个脉冲型/序列型)。鲍曼不动杆菌ST91是一个同时携带bla OXA-58和bla OXA-23的潜在的多药耐药风险克隆。BLA OXA-58定位在约52kb到约143kb之间。所有的质粒都可以电转化到鲍曼不动杆菌受体，但目前的复制子分型方案不能分型。在两个褐曲霉分离物、三个诺索康氏假丝酵母分离物和两个鲍曼假丝酵母分离物的含OXA-58的BLAOXA-58中发现了一种新的质粒复制酶，命名为epAci10。鉴定了BLAOXA-58的四种遗传背景。含有is6家族插入序列(isur1、is1008或is15)-ΔISAba3-like元件-bla OXA-58的转化子对碳青霉烯类抗生素不敏感，而结构完整的ISAba3类元件-bla OXA-58的转化子对碳青霉烯类抗生素敏感。本研究揭示了不动杆菌携带bla OXA-58质粒的独特特征。在中国菌株中，该菌株与不动杆菌属不同。产于欧洲国家。BLA OXA-58的遗传背景的多样性导致了不同的抗生素耐药性图谱。
Several studies have described the epidemiological distribution of bla OXA-58-harboring Acinetobacter baumannii in China. However, there is limited data concerning the replicon types of bla OXA-58-carrying plasmids and the genetic context surrounding bla OXA-58 in Acinetobacter spp. in China. Twelve non-duplicated bla OXA-58-harboring Acinetobacter spp. isolates were collected from six hospitals in five different cities between 2005 and 2010. The molecular epidemiology of the isolates was carried out using PFGE and multilocus sequence typing. Carbapenemase-encoding genes and plasmid replicase genes were identified by PCR. The genetic location of bla OXA-58 was analyzed using S1-nuclease method. Plasmid conjugation and electrotransformation were performed to evaluate the transferability of bla OXA-58-harboring plasmids. The genetic structure surrounding bla OXA-58 was determined by cloning experiments. The twelve isolates included two Acinetobacter pittii isolates (belong to one pulsotype), three Acinetobacter nosocomialis isolates (belong to two pulsotypes) and seven Acinetobacter baumannii isolates (belong to two pulsotypes/sequence types). A. baumannii ST91 was found to be a potential multidrug resistant risk clone carrying both bla OXA-58 and bla OXA-23. bla OXA-58 located on plasmids varied from ca. 52 kb to ca. 143 kb. All plasmids can be electrotransformed to A. baumannii recipient, but were untypeable by the current replicon typing scheme. A novel plasmid replicase named repAci10 was identified in bla OXA-58-harboring plasmids of two A. pittii isolates, three A. nosocomialis isolates and two A. baumannii isolates. Four kinds of genetic contexts of bla OXA-58 were identified. The transformants of plasmids with structure of IS6 family insertion sequence (ISOur1, IS1008 or IS15)-ΔISAba3-like element-bla OXA-58 displayed carbapenem nonsusceptible, while others with structure of intact ISAba3-like element-bla OXA-58 were carbapenem susceptible. The study revealed the unique features of bla OXA-58-carrying plasmids in Acinetobacter spp. in China, which were different from that of Acinetobacter spp. found in European countries. The diversity of the genetic contexts of bla OXA-58 contributed to various antibiotics resistance profiles.
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影响因子：
4.9
作者：
Chen, Te-Li;Wu, Roy Chen-Chih;Cho, Wen-Long
通讯作者：
Cho, Wen-Long
影响因子：
14.2
作者：
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通讯作者：
Visca, P.
影响因子：
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作者：
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通讯作者：
Vila, Jordi
影响因子：
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作者：
MARTIN, P;JULLIEN, E;COURVALIN, P
通讯作者：
COURVALIN, P
影响因子：
4.9
作者：
Poirel, L;Marqué, S;Nordmann, P
通讯作者：
Nordmann, P