RNA-containing cytoplasmic inclusion bodies in ciliated bronchial epithelium months to years after acute Kawasaki disease.
RNA-containing cytoplasmic inclusion bodies in ciliated bronchial epithelium months to years after acute Kawasaki disease.
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DOI：
10.1371/journal.pone.0001582
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发表时间：
2008-02-13
期刊：
影响因子：
3.7
通讯作者：
Orenstein, Jan M.
中科院分区：
文献类型：
--
作者：
Rowley, Anne H.;Baker, Susan C.;Shulman, Stanford T.;Garcia, Francesca L.;Fox, Linda M.;Kos, Ian M.;Crawford, Susan E.;Russo, Pierre A.;Hammadeh, Rashid;Takahashi, Kei;Orenstein, Jan M.
川崎病（KD）是发达国家儿童获得性心脏病的最常见原因。KD的病原尚不清楚，但可能是一种普遍存在的微生物，通常会引起无症状的儿童感染，仅在遗传易感个体中导致KD。从KD动脉组织中流行的IgA基因序列制备的KD合成抗体可检测急性KD中类似病毒ICI的胞浆内包涵体（ICI），但不能控制婴儿纤毛支气管上皮。除非持续感染是常见的，否则在晚期KD死亡患者和非KD疾病的老年人中，ICI的患病率应该很低。通过光镜检查晚期KD死亡患者和非婴儿对照组的肺组织是否存在ICI。对ICI阳性组织进行核酸染色和透射电镜（TEM）检查。在9-84岁的晚期KD死亡者中，有6/7（86%）和7/27（26%）的对照组中，纤毛支气管上皮存在ICI （p = 0.01）。核酸染色显示ICI内为RNA而非DNA。在一些KD病例的肺巨噬细胞中也发现了ICI。KD病例支气管上皮和巨噬细胞透射电镜显示细颗粒状均匀ICI。这些发现与一种以前未发现的、普遍存在的RNA病毒一致，这种病毒形成ICI，并可导致支气管上皮和巨噬细胞持续感染，是KD的病因。
Kawasaki Disease (KD) is the most common cause of acquired heart disease in children in developed nations. The KD etiologic agent is unknown but likely to be a ubiquitous microbe that usually causes asymptomatic childhood infection, resulting in KD only in genetically susceptible individuals. KD synthetic antibodies made from prevalent IgA gene sequences in KD arterial tissue detect intracytoplasmic inclusion bodies (ICI) resembling viral ICI in acute KD but not control infant ciliated bronchial epithelium. The prevalence of ICI in late-stage KD fatalities and in older individuals with non-KD illness should be low, unless persistent infection is common. Lung tissue from late-stage KD fatalities and non-infant controls was examined by light microscopy for the presence of ICI. Nucleic acid stains and transmission electron microscopy (TEM) were performed on tissues that were strongly positive for ICI. ICI were present in ciliated bronchial epithelium in 6/7 (86%) late-stage KD fatalities and 7/27 (26%) controls ages 9–84 years (p = 0.01). Nucleic acid stains revealed RNA but not DNA within the ICI. ICI were also identified in lung macrophages in some KD cases. TEM of bronchial epithelium and macrophages from KD cases revealed finely granular homogeneous ICI. These findings are consistent with a previously unidentified, ubiquitous RNA virus that forms ICI and can result in persistent infection in bronchial epithelium and macrophages as the etiologic agent of KD.
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影响因子：
6.4
作者：
Rowley, AH;Baker, SC;Crawford, SE
通讯作者：
Crawford, SE
影响因子：
6.4
作者：
Rowley, AH;Baker, SC;Orenstein, JM
通讯作者：
Orenstein, JM
影响因子：
3.7
作者：
Chatziandreou, N;Young, D;Randall, RE
通讯作者：
Randall, RE
DOI：
10.1164/rccm.200604-489oc
发表时间：
2006-12-15
影响因子：
24.7
作者：
Kaiser, Laurent;Aubert, John-David;Soccal, Paola M.
通讯作者：
Soccal, Paola M.
影响因子：
64.8
作者：
MUCHMORE, HG;PARKINSON, AJ;MILES, JAR
通讯作者：
MILES, JAR