New microsatellite markers for examining genetic variation in peripheral and core populations of the Coastal Giant Salamander (Dicamptodon tenebrosus).
New microsatellite markers for examining genetic variation in peripheral and core populations of the Coastal Giant Salamander (Dicamptodon tenebrosus).
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DOI：
10.1371/journal.pone.0014333
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发表时间：
2010-12-15
期刊：
影响因子：
3.7
通讯作者：
Richardson JS
中科院分区：
文献类型：
--
作者：
Dudaniec RY;Storfer A;Spear SF;Richardson JS
在加拿大不列颠哥伦比亚省，沿海大鲵（Dicamptodon tenebrosus）在其活动范围的北方边缘受到威胁，主要是由于林业措施和栖息地破碎化。因此，分散行为和人口的连接特性是该地区的一个优先事项，而在核心与周边地区的遗传分化仍然没有研究，在这个广泛的物种。我们提出了7个新的多态性微卫星标记用于群体遗传分析。黑暗我们研究了12个流中的基因座特征和遗传变异在该物种的北方范围限制在公元前，并在两个区域内代表次周边（北瀑布）和核心地区（南瀑布）在华盛顿州，美国。在BC中，每个位点的等位基因数范围为2-5，观察到的杂合度范围为0.044-0.825。遗传分化是最高的BC和南瀑布，BC和北瀑布之间的中间。在基因座，平均等位基因丰富度是相似的跨区域，而私人等位基因丰富度是最高的核心地区（校正样本量）。这些新的微卫星位点将是一个有价值的除了现有的标记，详细的景观和群体遗传分析的D。在它的分布范围内有黑腹龙。
The Coastal Giant Salamander (Dicamptodon tenebrosus) is classified as threatened at the northern periphery of its range in British Columbia (BC), Canada, primarily due to forestry practices and habitat fragmentation. Characterising dispersal behaviour and population connectivity is therefore a priority for this region, while genetic differentiation in core versus peripheral locations remains unstudied in this wide-ranging species. We present seven new polymorphic microsatellite markers for use in population genetic analyses of D. tenebrosus. We examine locus characteristics and genetic variation in 12 streams at the species' northern range limit in BC, and within two regions representing sub-peripheral (North Cascades) and core localities (South Cascades) in Washington State, United States. In BC, the number of alleles per locus ranged from 2–5 and observed heterozygosity ranged from 0.044–0.825. Genetic differentiation was highest between BC and the South Cascades, and intermediate between BC and the North Cascades. Across loci, mean allelic richness was similar across regions, while private allelic richness was highest in the core locality (corrected for sample size). These new microsatellite loci will be a valuable addition to existing markers for detailed landscape and population genetic analyses of D. tenebrosus across its range.
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影响因子：
4.9
作者：
Curtis, JM;Taylor, EB
通讯作者：
Taylor, EB
影响因子：
4.9
作者：
Gautschi, B;Tenzer, I;Schmid, B
通讯作者：
Schmid, B
影响因子：
3.3
作者：
Wang, JL
通讯作者：
Wang, JL
DOI：
10.1111/j.1471-8286.2004.00684.x
发表时间：
2004-09-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Van Oosterhout, C;Hutchinson, WF;Shipley, P
通讯作者：
Shipley, P
影响因子：
5.9
作者：
Curtis, JMR;Taylor, EB
通讯作者：
Taylor, EB