Ratiometric Reactive Oxygen Species Nanoprobe for Noninvasive In Vivo Imaging of Subcutaneous Inflammation/Infection.
Ratiometric Reactive Oxygen Species Nanoprobe for Noninvasive In Vivo Imaging of Subcutaneous Inflammation/Infection.
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DOI：
10.1166/jbn.2016.2268
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发表时间：
2016-08
影响因子：
2.9
通讯作者：
Hu W
中科院分区：
文献类型：
--
作者：
Zhou J;Weng H;Huang Y;Gu Y;Tang L;Hu W
活性氧（ROS）的释放伴随着急性炎症和感染，往往导致细胞死亡和组织损伤。近年来研究了几种活性氧活性生物发光探针来检测体内活性氧活性。不幸的是，这些探针不能用于量化ROS活性和炎症反应的程度，因为生物发光信号的程度也依赖于探针的浓度。为了解决这一挑战，我们制造了一种比例ROS探针，其中ROS敏感的化学发光剂和ROS不敏感的荧光参考染料被共轭到颗粒载体上。计算生物发光/参考荧光强度比，以反映局部ROS活性的程度，同时绕过与ROS探针浓度相关的生物发光强度变化。对比例探针的理化性质进行了表征。此外，我们评估了探针在体外检测ROS的准确性和重复性。比例探针检测炎症/感染组织中ROS生成的能力也通过炎症和感染的动物模型进行了检测。总体结果表明，比例ROS探针可以快速、无创地实时检测和量化伤口炎症反应和细菌感染的程度。
Release of reactive oxygen species (ROS) accompanied with acute inflammation and infection often results in cell death and tissue injury. Several ROS-reactive bioluminescent probes have been investigated in recent years to detect ROS activity in vivo. Unfortunately, these probes cannot be used to quantify the degree of ROS activity and inflammatory responses due to the fact that the extent of the bioluminescent signals is also probe-concentration dependent. To address this challenge, we fabricated a ratiometric ROS probe in which both ROS-sensitive chemiluminescent agents and ROS-insensitive fluorescent reference dye were conjugated to particle carriers. The bioluminescence/reference fluorescence intensity ratios was calculated to reflect the extent of localized ROS activities while circumventing the variations in bioluminescent intensities associated with the ROS probe concentrations. The physical and chemical properties of the ratiometric probes were characterized. Furthermore, we assessed the accuracy and reproducibility of the probe in detecting ROS in vitro. The ability of the ratiometric probes to detect ROS production in inflamed/infected tissues was also examined using animal models of inflammation and infection. The overall results imply that ratiometric ROS probes can rapidly and non-invasively detect and quantify the extent of inflammatory responses and bacterial infection on wounds in real time.
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影响因子：
7.4
作者：
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影响因子：
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DOI：
10.1073/pnas.0702427104
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