A Single Bout of Electroacupuncture Remodels Epigenetic and Transcriptional Changes in Adipose Tissue in Polycystic Ovary Syndrome.
A Single Bout of Electroacupuncture Remodels Epigenetic and Transcriptional Changes in Adipose Tissue in Polycystic Ovary Syndrome.
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DOI：
10.1038/s41598-017-17919-5
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发表时间：
2018-01-30
影响因子：
4.6
通讯作者：
Stener-Victorin E
中科院分区：
文献类型：
--
作者：
Kokosar M;Benrick A;Perfilyev A;Nilsson E;Källman T;Ohlsson C;Ling C;Stener-Victorin E
单次电针会导致多囊卵巢综合征（PCOS）妇女的肌肉收缩和全身葡萄糖摄取增加。多囊卵巢综合征患者脂肪组织中存在转录和表观遗传改变，我们假设电针可诱导表观遗传和转录改变以恢复代谢改变。21名患有PCOS的妇女接受了单次电针治疗，这增加了全身葡萄糖的摄取。在皮下脂肪组织活检中，我们确定了2369个基因（Q <0.05）的治疗诱导表达变化和7055个基因（Q = 0.11）的DNA甲基化变化。观察到FOSB（2405%）的表达增加最多，LOC 100128899（54%）的表达减少最多。最丰富的途径包括急性期反应信号传导和LXR/RXR激活。DNA甲基化变化范围为1 - 16%，407个甲基化位点与基因表达相关。在已知的PCOS差异表达基因中，电针逆转了80个基因的表达，包括PPAR γ和ADIPOR 2。Nr4a2和Junb表达的变化在大鼠中被肾上腺素能阻断剂逆转，表明基因表达的变化部分是由于交感神经系统的激活。总之，低频电针与肌肉收缩重塑引起代谢改善的表观遗传和转录变化。
A single bout of electroacupuncture results in muscle contractions and increased whole body glucose uptake in women with polycystic ovary syndrome (PCOS). Women with PCOS have transcriptional and epigenetic alterations in the adipose tissue and we hypothesized that electroacupuncture induces epigenetic and transcriptional changes to restore metabolic alterations. Twenty-one women with PCOS received a single bout of electroacupuncture, which increased the whole body glucose uptake. In subcutaneous adipose tissue biopsies, we identified treatment-induced expression changes of 2369 genes (Q < 0.05) and DNA methylation changes of 7055 individual genes (Q = 0.11). The largest increase in expression was observed for FOSB (2405%), and the largest decrease for LOC100128899 (54%). The most enriched pathways included Acute phase response signaling and LXR/RXR activation. The DNA methylation changes ranged from 1–16%, and 407 methylation sites correlated with gene expression. Among genes known to be differentially expressed in PCOS, electroacupuncture reversed the expression of 80 genes, including PPARγ and ADIPOR2. Changes in the expression of Nr4a2 and Junb are reversed by adrenergic blockers in rats demonstrating that changes in gene expression, in part, is due to activation of the sympathetic nervous system. In conclusion, low-frequency electroacupuncture with muscle contractions remodels epigenetic and transcriptional changes that elicit metabolic improvement.
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影响因子：
16.6
作者：
Hughes, Travis S.;Giri, Pankaj Kumar;de Vera, Ian Mitchelle S.;Marciano, David P.;Kuruvilla, Dana S.;Shin, Youseung;Blayo, Anne-Laure;Kamenecka, Theodore M.;Burris, Thomas P.;Griffin, Patrick R.;Kojetin, Douglas J.
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Kojetin, Douglas J.
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影响因子：
16.6
作者：
Cheng, Jingdong;Yang, Huirong;Fang, Jian;Ma, Lixiang;Gong, Rui;Wang, Ping;Li, Ze;Xu, Yanhui
通讯作者：
Xu, Yanhui
影响因子：
3.7
作者：
Amouzou, Cacylde;Breuker, Cyril;Bisbal, Catherine
通讯作者：
Bisbal, Catherine
影响因子：
5.8
作者：
Coviello, Andrea D.;Sam, Susan;Dunaif, Andrea
通讯作者：
Dunaif, Andrea