A novel DNA polymerase inhibitor and a potent apoptosis inducer: 2-mono-O-acyl-3-O-(alpha-D-sulfoquinovosyl)-glyceride with stearic acid.
A novel DNA polymerase inhibitor and a potent apoptosis inducer: 2-mono-O-acyl-3-O-(alpha-D-sulfoquinovosyl)-glyceride with stearic acid.
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一种新型 DNA 聚合酶抑制剂和有效的细胞凋亡诱导剂：2-单-O-酰基-3-O-(α-D-磺基喹诺酰基)-甘油酯与硬脂酸。
DOI：
10.1016/s1570-9639(02)00521-6
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Y. Mizushina
中科院分区：
文献类型：
--
作者：
C. Murakami;T. Yamazaki;S. Hanashima;Shunya Takahashi;M. Takemura;S. Yoshida;K. Ohta;H. Yoshida;F. Sugawara;K. Sakaguchi;Y. Mizushina
磺基喹诺酮基二酰甘油中的磺基糖脂，包括立体异构体，是α和β的有效抑制剂。然而，由于含有两种硬脂酸的α构型(α-SQDG-C18)几乎不能穿透细胞，所以它没有细胞毒性作用。我们尝试并成功地以α-SQDG-C18为原料，经胰酶水解法合成了含硬脂酸的磺基喹诺酮单酰甘油(α-SQMG-C18)。α-SQMG-C18抑制DNA聚合酶活性，对肿瘤细胞的生长有明显的抑制作用。α-SQMG-C18使细胞周期停滞于G1期，并诱导严重的细胞凋亡。α-SqMG-C18通过P53依赖的细胞凋亡途径诱导细胞死亡，与P53和细胞周期蛋白E表达增加有关。
Sulfo-glycolipids in the class of sulfoquinovosyl diacylglycerol (SQDG) including the stereoisomers are potent inhibitors of DNA polymerase α and β. However, since the α-configuration of SQDG with two stearic acids (α-SQDG-C18) can hardly penetrate cells, it has no cytotoxic effect. We tried and succeeded in making a permeable form, sulfoquinovosyl monoacylglycerol with a stearic acid (α-SQMG-C18) from α-SQDG-C18by hydrolysis with a pancreatic lipase. α-SQMG-C18inhibited DNA polymerase activity and was found to be a potent inhibitor of the growth of NUGC-3 cancer cells. α-SQMG-C18arrested the cell cycle at the G1 phase, and subsequently induced severe apoptosis. The arrest was correlated with an increased expression of p53 and cyclin E, indicating that α-SQMG-C18induced cell death through a p53-dependent apoptotic pathway.
影响因子：
56.9
作者：
DULIC, V;LEES, E;REED, SI
通讯作者：
REED, SI
DOI：
10.1073/pnas.89.16.7491
发表时间：
1992-08-15
影响因子：
11.1
作者：
KUERBITZ, SJ;PLUNKETT, BS;KASTAN, MB
通讯作者：
KASTAN, MB
细胞凋亡过程中 DNA 损伤信号激活细胞周期蛋白 E 依赖性激酶。
DOI：
10.1006/bbrc.1995.2353
发表时间：
1995
期刊：
Biochemical and biophysical research communications.
影响因子：
--
作者：
Dou,QP;An,B;Yu,C
通讯作者：
Yu,C