Geminiviruses encode additional small proteins with specific subcellular localizations and virulence function.
Geminiviruses encode additional small proteins with specific subcellular localizations and virulence function.
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DOI：
10.1038/s41467-021-24617-4
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发表时间：
2021-07-13
影响因子：
16.6
通讯作者：
Zhou X
中科院分区：
文献类型：
--
作者：
Gong P;Tan H;Zhao S;Li H;Liu H;Ma Y;Zhang X;Rong J;Fu X;Lozano-Durán R;Li F;Zhou X
双生病毒是一种编码能力有限的植物病毒。双生病毒编码的蛋白质传统上通过应用10 kDa的任意阈值来识别;然而，越来越清楚的是，小蛋白质在生物系统中发挥相关作用，这要求重新考虑这一标准。在这里，我们表明，双生病毒基因组含有额外的ORF。使用番茄黄化曲叶病毒，我们证明了这些小的ORF中的一些在感染过程中表达，并且编码的蛋白质显示特定的亚细胞定位。我们证明，这些额外的ORF中最大的，我们命名为V3，是所需的完全病毒感染，和V3蛋白定位在高尔基体和功能作为RNA沉默抑制。这些结果意味着双生病毒蛋白的库可以扩展，并且获得分子植物-双生病毒相互作用的全面概述将需要迄今被忽视的小ORF的详细研究。双生病毒是感染植物的小型病毒。在这里，作者筛选了双生病毒基因组中以前可能被忽视的小开放阅读框架，并显示其中一个编码一种名为V3的蛋白质，该蛋白质定位于高尔基体并作为RNA沉默抑制因子。
Geminiviruses are plant viruses with limited coding capacity. Geminivirus-encoded proteins are traditionally identified by applying a 10-kDa arbitrary threshold; however, it is increasingly clear that small proteins play relevant roles in biological systems, which calls for the reconsideration of this criterion. Here, we show that geminiviral genomes contain additional ORFs. Using tomato yellow leaf curl virus, we demonstrate that some of these small ORFs are expressed during the infection, and that the encoded proteins display specific subcellular localizations. We prove that the largest of these additional ORFs, which we name V3, is required for full viral infection, and that the V3 protein localizes in the Golgi apparatus and functions as an RNA silencing suppressor. These results imply that the repertoire of geminiviral proteins can be expanded, and that getting a comprehensive overview of the molecular plant-geminivirus interactions will require the detailed study of small ORFs so far neglected. Geminiviruses are small plant-infecting viruses. Here the authors screen the geminivirus genome for small open reading frames that may have been previously overlooked and show that one encodes a protein named V3 that localizes to the Golgi and acts as an RNA silencing suppressor.
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影响因子：
9.4
作者：
Li, Fangfang;Xu, Xiongbiao;Zhou, Xueping
通讯作者：
Zhou, Xueping
影响因子：
3.5
作者：
Luna, Ana P.;Morilla, Gabriel;Bejarano, Eduardo R.
通讯作者：
Bejarano, Eduardo R.
影响因子：
3.7
作者：
AZZAM, O;FRAZER, J;MAXWELL, DP
通讯作者：
MAXWELL, DP
影响因子：
11.4
作者：
Jouannet, Virginie;Moreno, Ana Beatriz;Maizel, Alexis
通讯作者：
Maizel, Alexis
DOI：
10.1073/pnas.0900604106
发表时间：
2009-06-23
影响因子：
11.1
作者：
Kosugi, Shunichi;Hasebe, Masako;Yanagawa, Hiroshi
通讯作者：
Yanagawa, Hiroshi