Synchrotron X-rays reveal the modes of Fe binding and trace metal storage in the brown algae Laminaria digitata and Ectocarpus siliculosus.
Synchrotron X-rays reveal the modes of Fe binding and trace metal storage in the brown algae Laminaria digitata and Ectocarpus siliculosus.
复制标题
同步加速器X射线揭示了棕色藻类laminaria digitata和Ectocarpus硅的Fe结合和痕量金属存储的模式。
DOI：
10.1093/mtomcs/mfad058
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发表时间：
2023-10-04
期刊：
影响因子：
3.4
通讯作者：
Kupper, Frithjof C.
中科院分区：
文献类型：
--
作者：
Mijovilovich, Ana;Cloetens, Peter;Lanzirotti, Antonio;Newville, Matt;Wellenreuther, Gerd;Kumari, Puja;Katsaros, Christos;Carrano, Carl J.;Kupper, Hendrik;Kupper, Frithjof C.
铁在海带中以非铁蛋白核的共塑方式积累，这似乎是褐藻的一个普遍特征。微探针研究表明，铁的结合取决于组织类型。海洋通常是一个缺铁的环境，近年来人们认识到褐藻具有独特的、不含铁蛋白的铁储存系统。以海带（Laminaria digitata）和丝状褐藻Ectocarpus siliculosus为研究对象，采用x射线微探针成像技术和纳米探针x射线荧光成像技术研究其铁、砷、锶和锌的定位，采用微x射线吸收近边结构（μXANES）研究其铁的结合。两种藻类的冷冻水合环境样品中铁的分布均表现出较高的皮层富集，具有共塑亚细胞定位。这应该在最近通过冷冻固定-冷冻替代的超微结构研究中看到，该研究发现了一种新型池，它可能具有储存功能，但与传统化学固定发现的外胞铁积累不同。锌在硅藻中的分布与铁共定位，而主要分布在数字藻的髓质中，这与As和Sr相似，As和Sr都主要存在于两种藻类的细胞壁中。XANES光谱表明，由于缺乏铁蛋白编码基因，铁在L. digitata中储存在矿物非铁蛋白核心中。我们发现，指草皮层主要含有一种类似铁蛋白的矿物质，而分生层可能包含一种额外的成分。纳米探针XRF层析成像显示棕藻（海带）海带藻（Laminaria digitata）菌体截面中锶的分布。
Iron is accumulated symplastically in kelp in a non-ferritin core that seems to be a general feature of brown algae. Microprobe studies show that Fe binding depends on tissue type. The sea is generally an iron-poor environment and brown algae were recognized in recent years for having a unique, ferritin-free iron storage system. Kelp (Laminaria digitata) and the filamentous brown alga Ectocarpus siliculosus were investigated using X-ray microprobe imaging and nanoprobe X-ray fluorescence tomography to explore the localization of iron, arsenic, strontium, and zinc, and micro-X-ray absorption near-edge structure (μXANES) to study Fe binding. Fe distribution in frozen hydrated environmental samples of both algae shows higher accumulation in the cortex with symplastic subcellular localization. This should be seen in the context of recent ultrastructural insight by cryofixation–freeze substitution that found a new type of cisternae that may have a storage function but differs from the apoplastic Fe accumulation found by conventional chemical fixation. Zn distribution co-localizes with Fe in E. siliculosus, whereas it is chiefly located in the L. digitata medulla, which is similar to As and Sr. Both As and Sr are mostly found at the cell wall of both algae. XANES spectra indicate that Fe in L. digitata is stored in a mineral non-ferritin core, due to the lack of ferritin-encoding genes. We show that the L. digitata cortex contains mostly a ferritin-like mineral, while the meristoderm may include an additional component. Distribution of Strontium in a cross-section of a thallus of the brown alga (kelp) Laminaria digitata, visualized by nanoprobe XRF tomography.
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