The KRÜPPEL-like transcription factor DATILÓGRAFO is required in specific cholinergic neurons for sexual receptivity in Drosophila females.
The KRÜPPEL-like transcription factor DATILÓGRAFO is required in specific cholinergic neurons for sexual receptivity in Drosophila females.
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DOI：
10.1371/journal.pbio.1001964
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发表时间：
2014-10
期刊：
影响因子：
9.8
通讯作者：
Sousa-Neves R
中科院分区：
文献类型：
--
作者：
Schinaman JM;Giesey RL;Mizutani CM;Lukacsovich T;Sousa-Neves R
果蝇的雌性决策需要在离散脑区的少量胆碱能神经元中表达一种转录因子。求偶是一种普遍的行为，一种性别向另一种性别传递一系列关于他们的物种身份、性别和作为配偶的适宜性的线索。在许多物种中，雌性破译雄性的这些表现，接受或拒绝它们。尽管求偶行为已经被研究了很长时间，但允许雌性产生这些互斥反应的基因和电路在很大程度上仍然未知。在这里，我们提供的证据表明kr<s:1> ppel样转录因子datilógrafo （dati）是成年雌性果蝇正常运动和求爱接受所必需的。Dati突变的雌性完全无法理解雄性的求偶行为，几乎总是拒绝雄性。分子分析表明，dati在大脑中广泛表达，其在兴奋性胆碱能神经元中的特异性去除再现了雌性求偶行为表型，而不是运动缺陷，表明这是两个可分离的功能。女性大脑的克隆分析确定了三个离散的焦点，其中需要数据来产生接受。这些神经元包括触角叶周围的神经元、外侧角周围的神经元和后上外侧原脑周围的神经元。总之，这些结果表明，数据是组织和维持大脑中一个相对简单的兴奋回路所必需的，这个回路允许雌性接受或拒绝雄性的求爱。雄性黑腹果蝇会发出一系列求爱信号，传递视觉、听觉和嗅觉信息，雌性必须解码这些信息，才能接受或拒绝交配。尽管女性在性选择中起着核心作用，但对于基因和神经回路是如何产生这种行为的，人们知之甚少。在这里，我们表明转录因子datilografo （dati）是组织和维持中枢大脑接受所需的神经回路所必需的。引人注目的是，数据在一个兴奋性回路中是必需的，该回路涉及少数神经元，这些神经元将乙酰胆碱作为神经递质表达，并位于嗅觉叶中，嗅觉叶是大脑中气味处理的第一个入口。此外，数据在另外两个大脑中心也是必需的：一个是嗅觉和可能是其他感官整合的区域，另一个是新的区域。综上所述，这些结果表明，一个复杂的行为可以由很少的兴奋性神经元产生，这表明接受和拒绝之间的明显界限可能涉及几十年前假设的不同的刺激阈值。
Female decision-making in Drosophila flies requires the expression of a transcription factor in a small number of cholinergic neurons in discrete brain regions. Courtship is a widespread behavior in which one gender conveys to the other a series of cues about their species identity, gender, and suitability as mates. In many species, females decode these male displays and either accept or reject them. Despite the fact that courtship has been investigated for a long time, the genes and circuits that allow females to generate these mutually exclusive responses remain largely unknown. Here, we provide evidence that the Krüppel-like transcription factor datilógrafo (dati) is required for proper locomotion and courtship acceptance in adult Drosophila females. dati mutant females are completely unable to decode male courtship and almost invariably reject males. Molecular analyses reveal that dati is broadly expressed in the brain and its specific removal in excitatory cholinergic neurons recapitulates the female courtship behavioral phenotype but not the locomotor deficits, indicating that these are two separable functions. Clonal analyses in female brains identified three discrete foci where dati is required to generate acceptance. These include neurons around the antennal lobe, the lateral horn, and the posterior superior lateral protocerebrum. Together, these results show that dati is required to organize and maintain a relatively simple excitatory circuit in the brain that allows females to either accept or reject courting males. Males of the fruit fly Drosophila melanogaster generate a series of courtship displays that convey visual, auditory, and olfactory information that females must decode in order to accept or reject mating. Despite the central role of female decision in sexual selection, relatively little is known about how genes and neural circuits generate this behavior. Here we show that the transcription factor datilografo (dati) is required to organize and maintain the neural circuitry required for acceptance in the central brain. Strikingly, dati is required in an excitatory circuit involving few neurons that express acetylcholine as their neurotransmitter and are located in the olfactory lobe, the first entry point for odor processing in the brain. In addition, dati is required in two other brain centers: a region where olfaction and presumably other senses are integrated and a novel region. Together these results show that a complex behavior can be generated by very few excitatory neurons, suggesting that the sharp cutoffs between acceptance and rejection may involve different thresholds of stimulation as postulated decades ago.
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影响因子：
3.3
作者：
BASTOCK, M
通讯作者：
BASTOCK, M
DOI：
10.1144/sp286.10
发表时间：
2007-01-01
期刊：
RISE AND FALL OF THE EDIACARAN BIOTA
影响因子：
--
作者：
Braun, A.;Chen, J.;Maas, A.
通讯作者：
Maas, A.
影响因子：
64.8
作者：
Jefferis, GSXE;Marin, EC;Luo, LQ
通讯作者：
Luo, LQ
影响因子：
1.5
作者：
Hayashi, S;Ito, K;Goto, S
通讯作者：
Goto, S
DOI：
10.1163/156853955x00184
发表时间：
1955-01-01
期刊：
BEHAVIOR
影响因子：
--
作者：
BASTOCK, M.;MANNING, A.
通讯作者：
MANNING, A.