Comparative molecular evolution of chitinases in ascomycota with emphasis on mycoparasitism lifestyle.
Comparative molecular evolution of chitinases in ascomycota with emphasis on mycoparasitism lifestyle.
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子囊菌几丁质酶的比较分子进化，重点是真菌寄生生活方式
DOI：
10.1099/mgen.0.000646
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发表时间：
2021-09
影响因子：
3.9
通讯作者：
Zhuang WY
中科院分区：
文献类型：
--
作者：
Wang C;Zeng ZQ;Zhuang WY
几丁质酶参与真菌生活史的多个方面，如细胞壁重塑、几丁质降解和真菌生活方式。为了提高我们对子囊菌门几丁质酶分子进化的认识，我们对该门72个代表性的几丁质酶基因家族进行了鉴定，并进行了系统发育、进化轨迹和选择压力分析。系统发育分析表明，几丁质酶基因家族大小和酶类型沿着物种进化而发生显著变化，尤其是B和C群。此外，在C组几丁质酶中还发现了两个新的亚组，C3和C4。随机出生和死亡的测试表明，基因扩增和收缩发生在大多数的类群，特别是肉座菌目（类Sordariomycetes）的物种。从酶功能的角度，我们推测A组几丁质酶主要参与物种的生长发育，而B组几丁质酶基因的扩增与真菌的真菌寄生和昆虫致病能力有关，C组几丁质酶基因的扩增在一定程度上可能与某些植物病原真菌的寄主范围扩大有关。进一步的选择压力测试表明，几丁质酶及其相关的氨基酸位点在进化过程中处于正选择状态，特别是在具有共同祖先的节位和肉座菌目的末端分支。这些结果合理地解释了子囊菌中几丁质酶基因的大小和功能差异，并为了解几丁质酶的进化轨迹，特别是向真菌寄生生活方式的进化轨迹提供了科学依据。
Chitinases are involved in multiple aspects of fungal life cycle, such as cell wall remodelling, chitin degradation and mycoparasitism lifestyle. To improve our knowledge of the chitinase molecular evolution of Ascomycota, the gene family of 72 representatives of this phylum was identified and subjected to phylogenetic, evolution trajectory and selective pressure analyses. Phylogenetic analysis showed that the chitinase gene family size and enzyme types varied significantly, along with species evolution, especially for groups B and C. In addition, two new subgroups, C3 and C4, are recognized in group C chitinases. Random birth and death testing indicated that gene expansion and contraction occurred in most of the taxa, particularly for species in the order Hypocreales (class Sordariomycetes). From an enzyme function point of view, we speculate that group A chitinases are mainly involved in species growth and development, while the expansion of genes in group B chitinases is related to fungal mycoparasitic and entomopathogenic abilities, and, to a certain extent, the expansion of genes in group C chitinases seems to be correlated with the host range broadening of some plant-pathogenic fungi in Sordariomycetes. Further selection pressure testing revealed that chitinases and the related amino acid sites were under positive selection in the evolutionary history, especially at the nodes sharing common ancestors and the terminal branches of Hypocreales. These results give a reasonable explanation for the size and function differences of chitinase genes among ascomycetes, and provide a scientific basis for understanding the evolutionary trajectories of chitinases, particularly that towards a mycoparasitic lifestyle.
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影响因子：
12.3
作者：
Kubicek CP;Herrera-Estrella A;Seidl-Seiboth V;Martinez DA;Druzhinina IS;Thon M;Zeilinger S;Casas-Flores S;Horwitz BA;Mukherjee PK;Mukherjee M;Kredics L;Alcaraz LD;Aerts A;Antal Z;Atanasova L;Cervantes-Badillo MG;Challacombe J;Chertkov O;McCluskey K;Coulpier F;Deshpande N;von Döhren H;Ebbole DJ;Esquivel-Naranjo EU;Fekete E;Flipphi M;Glaser F;Gómez-Rodríguez EY;Gruber S;Han C;Henrissat B;Hermosa R;Hernández-Oñate M;Karaffa L;Kosti I;Le Crom S;Lindquist E;Lucas S;Lübeck M;Lübeck PS;Margeot A;Metz B;Misra M;Nevalainen H;Omann M;Packer N;Perrone G;Uresti-Rivera EE;Salamov A;Schmoll M;Seiboth B;Shapiro H;Sukno S;Tamayo-Ramos JA;Tisch D;Wiest A;Wilkinson HH;Zhang M;Coutinho PM;Kenerley CM;Monte E;Baker SE;Grigoriev IV
通讯作者：
Grigoriev IV
影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
影响因子：
3.2
作者：
Bolar, JP;Norelli, JL;Aldwinckle, HS
通讯作者：
Aldwinckle, HS
影响因子：
10.7
作者：
Janiak, Mareike C.;Chaney, Morgan E.;Tosi, Anthony J.
通讯作者：
Tosi, Anthony J.
DOI：
10.1073/pnas.91.23.10903
发表时间：
1994-11-08
影响因子：
11.1
作者：
CARSOLIO, C;GUTIERREZ, A;HERRERAESTRELLA, A
通讯作者：
HERRERAESTRELLA, A