Physiological, genomic and transcriptional diversity in responses to boron deficiency in rapeseed genotypes.
Physiological, genomic and transcriptional diversity in responses to boron deficiency in rapeseed genotypes.
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油菜基因型对缺硼反应的生理、基因组和转录多样性
DOI：
10.1093/jxb/erw342
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发表时间：
2016-10
影响因子：
6.9
通讯作者：
Xu F
中科院分区：
文献类型：
--
作者：
Hua Y;Zhou T;Ding G;Yang Q;Shi L;Xu F
对缺硼反应不同的油菜基因型的生理和转录变异以及遗传多样性进行了全面检查，发现转录组学辅助的 QTL-seq 分析可以加快具有复杂基因组的植物物种中数量性状基因的鉴定。异源四倍体油菜籽（甘蓝型油菜 L. AnAnCnCn，2n=4x=38）非常容易受到硼 (B) 缺乏的影响，硼 (B) 缺乏是一种广泛存在的限制因素，会导致种子产量严重损失。油菜基因型中发现的对 B 缺乏敏感性的遗传变异强调了复杂的反应结构。在本研究中，与 B 高效基因型‘青优 10’（‘QY10’）相比，B 低效基因型‘Westar 10’（‘W10’）通过活性氧过度积累、严重的脂质过氧化、明显的质壁分离、花器官发生异常和广泛的不育来应对营养和生殖发育过程中 B 的缺乏。 ‘QY10’和‘W10’的全基因组重测序（WGS）揭示了异源四倍体油菜基因组（~1130Mb）中共有 1 605 747 个单核苷酸多态性和 218 755 个插入/缺失不均匀分布。数字基因表达 (DGE) 分析发现，与维生素 B 转运蛋白、抗氧化酶以及细胞壁和细胞膜维持相关的基因更多，在维生素 B 缺乏的情况下，“QY10”根中的转录水平高于“W10”。此外，基于全基因组测序和对源自‘QY10’和‘W10’的双单倍体（DH）品系池的批量分离分析，在C2染色体上对B效率的两个显着数量性状位点（QTL）进行了表征，然后DGE辅助的QTL-seq分析确定了结节蛋白26样内在蛋白基因和ATP结合盒（ABC）转运蛋白基因作为调节B效率的相应候选基因。这项研究有助于更全面地了解 B 缺乏的差异生理和转录反应以及油菜基因型中丰富的遗传多样性，并且 DGE 辅助的 QTL-seq 分析为快速解析具有复杂基因组的植物物种的数量性状基因提供了新的见解。
A comprehensive examination is made of physiological and transcriptional variations and genetic diversity of rapeseed genotypes differing in their response to boron deficiency, and transcriptomics-assisted QTL-seq analyses are found to expedite the identification of quantitative trait genes in plant species with complex genomes. Allotetraploid rapeseed (Brassica napus L. AnAnCnCn, 2n=4x=38) is highly susceptible to boron (B) deficiency, a widespread limiting factor that causes severe losses in seed yield. The genetic variation in the sensitivity to B deficiency found in rapeseed genotypes emphasizes the complex response architecture. In this research, a B-inefficient genotype, ‘Westar 10’ (‘W10’), responded to B deficiencies during vegetative and reproductive development with an over-accumulation of reactive oxygen species, severe lipid peroxidation, evident plasmolysis, abnormal floral organogenesis, and widespread sterility compared to a B-efficient genotype, ‘Qingyou 10’ (‘QY10’). Whole-genome re-sequencing (WGS) of ‘QY10’ and ‘W10’ revealed a total of 1 605 747 single nucleotide polymorphisms and 218 755 insertions/deletions unevenly distributed across the allotetraploid rapeseed genome (~1130Mb). Digital gene expression (DGE) profiling identified more genes related to B transporters, antioxidant enzymes, and the maintenance of cell walls and membranes with higher transcript levels in the roots of ‘QY10’ than in ‘W10’ under B deficiency. Furthermore, based on WGS and bulked segregant analysis of the doubled haploid (DH) line pools derived from ‘QY10’ and ‘W10’, two significant quantitative trait loci (QTLs) for B efficiency were characterized on chromosome C2, and DGE-assisted QTL-seq analyses then identified a nodulin 26-like intrinsic protein gene and an ATP-binding cassette (ABC) transporter gene as the corresponding candidates regulating B efficiency. This research facilitates a more comprehensive understanding of the differential physiological and transcriptional responses to B deficiency and abundant genetic diversity in rapeseed genotypes, and the DGE-assisted QTL-seq analyses provide novel insights regarding the rapid dissection of quantitative trait genes in plant species with complex genomes.
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影响因子：
4.4
作者：
Huang S;Deng L;Guan M;Li J;Lu K;Wang H;Fu D;Mason AS;Liu S;Hua W
通讯作者：
Hua W
DOI：
10.1534/g3.115.021279
发表时间：
2016-04-07
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Fletcher RS;Herrmann D;Mullen JL;Li Q;Schrider DR;Price N;Lin J;Grogan K;Kern A;McKay JK
通讯作者：
McKay JK
影响因子：
5.4
作者：
Bu, Fengjiao;Chen, Huiming;Huang, Sanwen
通讯作者：
Huang, Sanwen
影响因子：
3.8
作者：
Choi JW;Choi BH;Lee SH;Lee SS;Kim HC;Yu D;Chung WH;Lee KT;Chai HH;Cho YM;Lim D
通讯作者：
Lim D
影响因子：
4.4
作者：
Hao QN;Zhou XA;Sha AH;Wang C;Zhou R;Chen SL
通讯作者：
Chen SL