The POU protein Oct-6 is a nucleocytoplasmic shuttling protein.
The POU protein Oct-6 is a nucleocytoplasmic shuttling protein.
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POU 蛋白 Oct-6 是一种核质穿梭蛋白。
DOI：
10.1093/nar/gki947
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发表时间：
2005
影响因子：
14.9
通讯作者：
Wegner, M
中科院分区：
文献类型：
--
作者：
Baranek, C;Sock, E;Wegner, M
与许多POU结构域蛋白一样，Oct-6在脊椎动物的发育过程中发挥着重要作用。与其在神经发生和髓鞘形成过程中作为转录调节因子的功能一致，Oct-6主要存在于细胞核中。核进口是由POU同源结构域N端的核定位信号介导的。这里我们显示，Oct-6还含有核输出信号，因此Oct-6能够不断地在细胞核和细胞质之间穿梭。这个核输出信号也定位在POU的同源结构域中，作为螺旋2和DNA识别螺旋3的连接环的一部分。它符合疏水的富含亮氨酸的输出序列的共识，并通过CRM1/Exp1介导核输出。一些氨基酸的替换或插入使这种核输出序列失活，轻微地减少了Oct-6与其八聚体识别元件的DNA结合，但强烈干扰了Oct-6依赖的转录激活，因此认为核输出和核质穿梭是Oct-6功能的重要方面。重要的是，识别出的Oct-6核输出信号在大多数其他脊椎动物POU蛋白中都是保守的。因此，核出口可能对POU蛋白在整个发育过程中的功能具有普遍意义。
Like many POU domain proteins, Oct-6 plays important roles during vertebrate development. In accord with its function as a transcriptional regulator during neurogenesis and myelination, Oct-6 is predominantly found in the nucleus. Nuclear import is mediated by a nuclear localization signal at the N-terminal end of the POU homeodomain. Here we show, that Oct-6 in addition contains a nuclear export signal so that Oct-6 is able to shuttle constantly between nucleus and cytoplasm. This nuclear export signal is also localized in the POU homeodomain as part of helix 2 and the connecting loop to DNA recognition helix 3. It conforms to the consensus of hydrophobic leucine-rich export sequences and mediates export from the nucleus via CRM1/Exp1. Several amino acid substitutions or insertions that inactivate this nuclear export sequence, reduce DNA-binding of Oct-6 to its octamer recognition element slighty, but interfere strongly with Oct-6-dependent transcriptional activation, thus arguing that nuclear export and nucleocytoplasmic shuttling are essential aspects of Oct-6 function. Importantly, the nuclear export signal identified for Oct-6 is conserved in most, if not all other vertebrate POU proteins. Nuclear export might therefore be of general relevance for POU protein function throughout development.
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影响因子：
64.5
作者：
Fornerod, M;Ohno, M;Mattaj, IW
通讯作者：
Mattaj, IW
DOI：
10.1083/jcb.145.2.255
发表时间：
1999-04-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Paraskeva E;Izaurralde E;Bischoff FR;Huber J;Kutay U;Hartmann E;Lührmann R;Görlich D
通讯作者：
Görlich D
影响因子：
5.3
作者：
Friedrich, RP;Schlierf, B;Wegner, M
通讯作者：
Wegner, M
DOI：
10.1073/pnas.89.8.3280
发表时间：
1992-04-15
影响因子：
11.1
作者：
HARA, Y;ROVESCALLI, AC;NIRENBERG, M
通讯作者：
NIRENBERG, M
影响因子：
4.8
作者：
Ikuta, T;Kobayashi, Y;Kawajiri, K
通讯作者：
Kawajiri, K