High-Dimensional Single-Cell Analysis Identifies Organ-Specific Signatures and Conserved NK Cell Subsets in Humans and Mice.
High-Dimensional Single-Cell Analysis Identifies Organ-Specific Signatures and Conserved NK Cell Subsets in Humans and Mice.
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高维单细胞分析确定了人和小鼠中器官特异性的特异性特异性特异性特异性特异性特异性特异性特异性标志和保守的NK细胞子集。
DOI：
10.1016/j.immuni.2018.09.009
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发表时间：
2018-11-20
期刊：
影响因子：
32.4
通讯作者：
Vivier E
中科院分区：
文献类型：
--
作者：
Crinier A;Milpied P;Escalière B;Piperoglou C;Galluso J;Balsamo A;Spinelli L;Cervera-Marzal I;Ebbo M;Girard-Madoux M;Jaeger S;Bollon E;Hamed S;Hardwigsen J;Ugolini S;Vély F;Narni-Mancinelli E;Vivier E
自然杀伤（NK）细胞是参与抗微生物和抗肿瘤应答的先天性淋巴样细胞（ILC）。在人类和小鼠中已经报道了几种NK细胞亚群，但它们在器官和物种之间的异质性仍然很差。我们通过对从脾脏和血液中分离的数千个单个细胞进行单细胞RNA测序来评估人类和小鼠NK细胞的多样性。无偏转录聚类显示两个不同的签名区分脾和血液NK细胞。该分析在单细胞分辨率下鉴定了小鼠脾脏中的三个亚群和人脾脏中的四个亚群，以及小鼠和人血液中各两个亚群。种内和种间转录组图谱的比较突出了两个主要亚群NK 1和NK 2在器官和种间的相似性。这种无偏见的方法提供了对NK细胞生物学的深入了解，并为将小鼠研究转化为人类生理学和疾病奠定了基础。脾和血液NK细胞上的scRNA-seq揭示了器官特异性特征scRNA-seq揭示了血液和脾NK细胞中NK细胞的异质性NK细胞上的scRNA-seq将NK 1定义为人CD 56 dim和小鼠CD 27-CD 11b + NK细胞NK细胞上的scRNA-seq将NK 2定义为人CD 56 bright和小鼠CD 27 + CD 11b-NK细胞已经在人和小鼠中报道了几种NK细胞亚群，但是它们的异质性仍然没有得到很好的表征。使用高通量单细胞RNA-seq，Crinier等人提供了来自脾和血液的NK细胞的保守的组织特异性基因特征，并鉴定了在器官和物种中转录相似的两个主要NK细胞亚群。
Natural killer (NK) cells are innate lymphoid cells (ILCs) involved in antimicrobial and antitumoral responses. Several NK cell subsets have been reported in humans and mice, but their heterogeneity across organs and species remains poorly characterized. We assessed the diversity of human and mouse NK cells by single-cell RNA sequencing on thousands of individual cells isolated from spleen and blood. Unbiased transcriptional clustering revealed two distinct signatures differentiating between splenic and blood NK cells. This analysis at single-cell resolution identified three subpopulations in mouse spleen and four in human spleen, and two subsets each in mouse and human blood. A comparison of transcriptomic profiles within and between species highlighted the similarity of the two major subsets, NK1 and NK2, across organs and species. This unbiased approach provides insight into the biology of NK cells and establishes a rationale for the translation of mouse studies to human physiology and disease. scRNA-seq on spleen and blood NK cells reveals organ-specific signatures scRNA-seq reveals the heterogeneity of NK cells in the blood and spleen scRNA-seq on NK cells defines NK1 as human CD56dim and mouse CD27−CD11b+ NK cells scRNA-seq on NK cells defines NK2 as human CD56bright and mouse CD27+CD11b− NK cells Several NK cell subsets have been reported in humans and mice, but their heterogeneity remains poorly characterized. Using high-throughput single-cell RNA-seq, Crinier et al. provide conserved tissue-specific gene signatures of NK cells from spleen and blood and identified two major NK cell subsets transcriptionally similar across organs and species.
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影响因子：
30.5
作者：
通讯作者：
--
影响因子：
17.1
作者：
Horowitz A;Strauss-Albee DM;Leipold M;Kubo J;Nemat-Gorgani N;Dogan OC;Dekker CL;Mackey S;Maecker H;Swan GE;Davis MM;Norman PJ;Guethlein LA;Desai M;Parham P;Blish CA
通讯作者：
Blish CA
DOI：
10.1126/science.1198687
发表时间：
2011-01-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Vivier E;Raulet DH;Moretta A;Caligiuri MA;Zitvogel L;Lanier LL;Yokoyama WM;Ugolini S
通讯作者：
Ugolini S
DOI：
10.1073/pnas.0609692104
发表时间：
2007-02-27
影响因子：
11.1
作者：
Walzer, Thierry;Blery, Mathieu;Vivier, Eric
通讯作者：
Vivier, Eric
影响因子：
32.4
作者：
Simoni Y;Fehlings M;Kløverpris HN;McGovern N;Koo SL;Loh CY;Lim S;Kurioka A;Fergusson JR;Tang CL;Kam MH;Dennis K;Lim TKH;Fui ACY;Hoong CW;Chan JKY;Curotto de Lafaille M;Narayanan S;Baig S;Shabeer M;Toh SES;Tan HKK;Anicete R;Tan EH;Takano A;Klenerman P;Leslie A;Tan DSW;Tan IB;Ginhoux F;Newell EW
通讯作者：
Newell EW