Correlative microscopy and block-face imaging (CoMBI): a 3D imaging method with wide applicability in the field of biological science
Correlative microscopy and block-face imaging (CoMBI): a 3D imaging method with wide applicability in the field of biological science
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相关显微镜和块面成像（CoMBI）：一种在生物科学领域具有广泛适用性的3D成像方法
DOI：
10.1007/s12565-023-00705-x
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发表时间：
2023
影响因子：
1.2
通讯作者：
Iwasaki Hirohide
中科院分区：
文献类型：
--
作者：
Tajika Yuki;Ishii Nobukazu;Morimura Yoshihiro;Fukuda Kyosuke;Shikada Mitsuaki;Murakami Tohru;Ichinose Sotaro;Yoshimoto Yuhei;Iwasaki Hirohide
相关显微镜和块面成像（Correlative microscopy and block-face imaging，CoMBI）是一种成像方法，其特征在于能够获得连续的块面图像作为三维（3D）数据集和用于二维（2D）光学显微镜分析的切片。这些3D和2D形态学数据可以相互关联以便于数据解释。CoMBI是一种易于安装和低成本的3D成像方法，因为它的系统可以由研究人员使用普通切片机，消费数码相机和一些自制设备组装，并且其安装和说明手册是开源的。在我们实验室首次发布CoMBI方法后，CoMBI系统已安装在十几个实验室中，用于各种生物标本的3D分析。CoMBI的典型应用是使用标本的自然颜色和对比度进行3D解剖分析。我们一直使用CoMBI分析人脑，以获得精细的三维解剖结构，作为确定神经系统疾病的原因和提高手术效果的参考。最近，我们一直在使用CoMBI检测色原的颜色，用于标记特定的分子。用CoMBI对小鼠胚胎进行了成像，并成功地可视化了X-gal的三维分布。因此，CoMBI现在可以用于许多目的，包括3D解剖分析，使用切片的2D显微镜和特定分子的3D分布。这表明CoMBI在生物学研究领域应该得到更广泛的应用。
Correlative microscopy and block-face imaging (CoMBI) is an imaging method, which is characterized by the ability to obtain both serial block-face images as a 3-dimentional (3D) dataset and sections for 2-dimentional (2D) light microscopic analysis. These 3D and 2D morphological data can be correlated with each other to facilitate data interpretation. CoMBI is an easy-to-install and low-cost 3D imaging method since its system can be assembled by the researcher using a regular microtome, consumer digital camera, and some self-made devices, and its installation and instruction manuals are open-source. After the first release of CoMBI method from our laboratory, CoMBI systems have been installed in more than a dozen laboratories and are used for 3D analysis of various biological specimens. Typical application of CoMBI is 3D anatomical analysis using the natural color and contrast of the specimen. We have been using CoMBI for analyzing human brain to obtain the fine 3D anatomy as a reference to determine the causes of neurological diseases and to improve the effectiveness of surgery. Recently, we have been using CoMBI for detecting the colors of chromogens, which are used for labeling specific molecules. Mouse embryos colored with X-gal, a conventional chromogen for detecting LacZ products, were imaged using CoMBI, and the 3D distribution of X-gal was successfully visualized. Thus, CoMBI can now be used for many purposes, including 3D anatomical analysis, 2D microscopy using sections, and 3D distribution of specific molecules. These suggest that CoMBI should be more widely used in the field of biological research.
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影响因子：
5.8
作者：
Wollman AJ;Nudd R;Hedlund EG;Leake MC
通讯作者：
Leake MC
高分辨率落射显微镜
DOI：
10.1088/978-0-7503-3059-6ch13
发表时间：
2021
期刊：
--
影响因子：
--
作者：
W. Weninger;S. Geyer
通讯作者：
S. Geyer
整体安装和块面成像的组合方法：从传统原位杂交中获取基因表达模式的 3D 数据
DOI：
--
发表时间：
2022
期刊：
Development, Growth and Differentiation
影响因子：
--
作者：
Aldy Anindyawan Sutrisno;Wataru Katano;Hayata Kawamura;Yuki Tajika;Kazuko Koshiba
通讯作者：
Kazuko Koshiba
影响因子：
30.8
作者：
Weninger, WJ;Mohun, T
通讯作者：
Mohun, T
影响因子：
2.5
作者：
Fedorov, Andriy;Beichel, Reinhard;Kalpathy-Cramer, Jayashree;Finet, Julien;Fillion-Robin, Jean-Christophe;Pujol, Sonia;Bauer, Christian;Jennings, Dominique;Fennessy, Fiona;Sonka, Milan;Buatti, John;Aylward, Stephen;Miller, James V.;Pieper, Steve;Kikinis, Ron
通讯作者：
Kikinis, Ron