Developmental patterns and characteristics of epicardial cell markers Tbx18 and Wt1 in murine embryonic heart.
Developmental patterns and characteristics of epicardial cell markers Tbx18 and Wt1 in murine embryonic heart.
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小鼠胚胎心脏心外膜细胞标记物Tbx18和Wt1的发育模式和特征
DOI：
10.1186/1423-0127-18-67
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发表时间：
2011-08-26
影响因子：
11
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Zeng B;Ren XF;Cao F;Zhou XY;Zhang J
尽管近期研究强调了心外膜细胞在心脏发育和再生过程中的作用，但由于心外膜细胞谱系追踪问题，其生心肌潜能仍存在争议。因此，本研究旨在检测Tbx18和Wt1在胚胎心脏中的表达，并确定Tbx18和Wt1自身是否在心肌细胞中表达。
在不同阶段收集小鼠胚胎心脏，分别用Tbx18和心脏转录因子Nkx2.5或威尔姆斯瘤1（Wt1）和Nkx2.5进行免疫荧光共染色。
Tbx18和Wt1（而非Nkx2.5）在前心外膜和心外膜中表达。从胚胎期第10.5天到至少第14.5天，Tbx18在心脏内的细胞中表达；这些表达Tbx18的细胞为Nkx2.5阳性，但在第14.5天有少数细胞为Nkx2.5阴性。从胚胎期第12.5天到至少第14.5天，Wt1在心脏内的细胞中表达，但这些表达Wt1的细胞为Nkx2.5阴性。
本研究获得的数据表明，从胚胎期第10.5天到至少第14.5天，Tbx18在心肌细胞中表达，从胚胎期第12.5天到至少第14.5天，Wt1在心脏内表达，但不在心肌细胞中表达。这些发现可能为心外膜细胞在心脏再生中的作用提供新的见解。
BackgroundAlthough recent studies have highlighted the role of epicardial cells during cardiac development and regeneration, their cardiomyogenic potential is still controversial due to the question of lineage tracing of epicardial cells. The present study therefore aimed to examine the the expression of Tbx18 and Wt1 in embryonic heart and to identify whether Tbx18 and Wt1 themselves expressed in the cardiomyocyte.MethodsMouse embryonic hearts were collected at different stages for immunofluorescence costaining with either Tbx18 and the cardiac transcription factor Nkx2.5 or Wilms tumor 1 (Wt1) and Nkx2.5.ResultsTbx18 and Wt1, but not Nkx2.5, were expressed in the proepicardium and epicardium. Tbx18 was expressed in cells within the heart from E10.5 to at least E14.5; these Tbx18-expressing cells were Nkx2.5 positive, except for a few cells that were Nkx2.5 negative at E14.5. Wt1 was expressed in cells within the heart from E12.5 to at least E14.5, but these Wt1-expressing cells were Nkx2.5 negative.ConclusionThe data obtained in this study demonstrate that Tbx18 is expressed in the cardiomyocytes from E10.5 to at least E14.5, and Wt1 is expressed within the heart from E12.5 to at least E14.5, but not in the cardiomyocyte. These findings may provide new insights on the role of the epicardial cells in cardiac regeneration.
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