Trypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.
Trypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.
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DOI：
10.1371/journal.pone.0051804
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Mortara RA
中科院分区：
文献类型：
--
作者：
Cruz MC;Souza-Melo N;da Silva CV;Darocha WD;Bahia D;Araújo PR;Teixeira SR;Mortara RA
Trypanosoma cruzi is a protozoan parasite that comprises different phylogenetic groups and is the causative agent of Chagas’ disease. Different T. cruzi strains present differences in infectivity in in vitro and in vivo experimental models, which are likely related to the expression of different virulence factors. Amastin is a surface glycoprotein abundantly expressed on the intracellular mammalian amastigote form of the parasite. In this study, we showed that a highly infective strain (G strain) of extracellular amastigote (EA) invasive forms expressed reduced RNA levels of amastin compared to a less infective strain (CL). The treatment of HeLa cells with recombinant δ-amastin reduced infectivity of EA forms. However, the ectopic expression of δ-amastin accelerated amastigote differentiation into trypomastigotes. Corroborating the virulence behavior in association with amastin expression, the EAs overexpressing amastin were precociously and robustly observed in the liver of susceptible mouse strains (A/JUnib), whereas parasitemia was never detected in in vivo assays. This is the first report on the regulatory role of amastin in the course of both in vitro and in vivo T. cruzi infection.
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影响因子：
--
作者：
Claser C;Curcio M;de Mello SM;Silveira EV;Monteiro HP;Rodrigues MM
通讯作者：
Rodrigues MM
影响因子：
5.5
作者：
Mackinnon, M. J.;Gandon, S.;Read, A. F.
通讯作者：
Read, A. F.
影响因子：
2.4
作者：
ANDERSON, RM;MAY, RM
通讯作者：
MAY, RM
影响因子：
5.8
作者：
da Silva, Claudio V.;Kawashita, Silvia Y.;Mortara, Renato A.
通讯作者：
Mortara, Renato A.
DOI：
10.1590/s0001-37652005000100006
发表时间：
2005-03-01
期刊：
Anais da Academia Brasileira de Ciências
影响因子：
--
作者：
Mortara, Renato A.;Andreoli, Walter K.;Silva, Solange da
通讯作者：
Silva, Solange da